College of Food Science and Technology, Bohai University, Jinzhou 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou 121013, China.
College of Food Science and Technology, Bohai University, Jinzhou 121013, China.
Food Chem. 2023 Feb 15;402:134235. doi: 10.1016/j.foodchem.2022.134235. Epub 2022 Sep 14.
Nitrofurans such as furaltadone and nitrofurantoin are a type of synthetic broad-spectrum antibiotics. Various fluorescent nanomaterials have been used as labeling materials in immunochromatographic assays (ICAs) for nitrofurans detection. However, previous fluorescent nanomaterials can undergo aggregation-caused quenching, leading to a decrease in the detection sensitivity. In this study, we developed a multiplex immunochromatographic assay (mICA) based on dual-color aggregation-induced emission nanoparticles (AIENPs) as signal labels for the simultaneous detection of 3-amino-5-morpholino-methyl-1,3-oxazolidinone (AMOZ) and 1-aminohydantoin (AHD), which were the metabolites of furaltadone and nitrofurantoin, respectively. Under optimal conditions, the cut-off values of the mICA for derivatized AMOZ (2-NP-AMOZ) and AHD (2-NP-AHD) reached up to 3 and 5 ng/mL, respectively. These values are at least 166-and 200-fold higher than those of the commercial gold nanoparticles (GNPs)-based test strip, respectively. Furthermore, the test strip was successfully applied to the samples, with acceptable recoveries in the range of 83.0-98.2%.
硝呋喃类如呋喃妥因和呋喃它酮是一类合成的广谱抗生素。各种荧光纳米材料已被用作免疫层析测定法（ICAs）中用于检测硝呋喃类的标记材料。然而，以前的荧光纳米材料可能会发生聚集诱导猝灭，导致检测灵敏度降低。在这项研究中，我们开发了一种基于双色聚集诱导发射纳米粒子（AIENPs）的多重免疫层析测定法（mICA），作为信号标记物，用于同时检测 3-氨基-5-吗啉基甲基-1,3-恶唑烷酮（AMOZ）和 1-氨基乙内酰脲（AHD），它们分别是呋喃妥因和呋喃它酮的代谢物。在最佳条件下，mICA 对衍生化 AMOZ（2-NP-AMOZ）和 AHD（2-NP-AHD）的截止值分别高达 3 和 5 ng/mL。这些值分别比商用金纳米粒子（GNPs）测试条高至少 166 倍和 200 倍。此外，该测试条成功应用于样品中，回收率在 83.0-98.2%范围内。
