Department of Histology and Embryology, School of Medicine, Dicle University, Diyarbakır, Turkey.
Pol J Vet Sci. 2022 Sep;25(3):447-454. doi: 10.24425/pjvs.2022.142029.
In this study, we aimed to investigate the effects of vitamin E on mouse adrenal glands in immobilization stress. Twenty-eight male, 10-week-old, BALB/C mice weighing 30-45 grams were divided into four groups. Mice were placed in a cage where no movement was allowed 6 hours/day for 7 days for immobilization stress. 10 ml/kg vitamin E was administered orogastrically 1 hour before immobilization stress in the vitamin E and stress+vitamin E group. At the end of the 7th day, all the animals were subjected to elevated-plus maze (anxiety) and forced swimming (depression) tests. Left adrenal glands were dissected for routine paraffin tissue embedding protocol. Adrenal sections were stained with hematoxylin-eosin and Azan. Malonaldehyde (MDA) levels were also measured in the adrenal tissues. Anxiety level (0.023), depression level (p=0.042) and MDA values (p=0.01) were significantly increased in the stress group. Histological sections of the stress group showed cortical atrophy, medullary hypertrophy, vascular dilation and hemorrhage. Azan staining revealed a thinned capsule and corticomedullary fibrosis in the stress group. Pathologies induced by immobilization stress were mostly reversed after vitamin E administration. The results suggested that vitamin E alleviates adverse effects of immobilization stress (oxidative, behavioral and histopathologic changes) in mice.
在这项研究中，我们旨在研究维生素 E 对束缚应激小鼠肾上腺的影响。将 28 只雄性、10 周龄、体重 30-45 克的 BALB/C 小鼠分为四组。将小鼠置于笼子中，每天允许其运动 6 小时，进行 7 天的束缚应激。在束缚应激前 1 小时，维生素 E 组和应激+维生素 E 组通过口服给予 10ml/kg 的维生素 E。在第 7 天结束时，所有动物都进行高架十字迷宫（焦虑）和强迫游泳（抑郁）测试。左肾上腺被解剖用于常规石蜡组织包埋方案。用苏木精-伊红和阿赞染色法对肾上腺切片进行染色。还测量了肾上腺组织中的丙二醛 (MDA) 水平。应激组的焦虑水平（0.023）、抑郁水平（p=0.042）和 MDA 值（p=0.01）显著升高。应激组的组织学切片显示皮质萎缩、髓质肥大、血管扩张和出血。阿赞染色显示应激组的包膜变薄和皮质髓质纤维化。束缚应激引起的病理变化在给予维生素 E 后大多得到逆转。结果表明，维生素 E 可减轻束缚应激（氧化、行为和组织病理学变化）对小鼠的不良影响。
