Kebede Megersa, Fite Tarekegn
School of Plant Sciences, College of Agriculture and Environmental Sciences, Haramaya University, Haramaya, Ethiopia.
Front Mol Biosci. 2022 Sep 7;9:944774. doi: 10.3389/fmolb.2022.944774. eCollection 2022.
The fall armyworm (Lepidoptera: Noctuidae) is among the invasive insect pests that damages maize and sorghum, the high-priority crops in newly colonized agro-ecologies, including African contexts. Owing to the increasing infestation of the pest and the limitations of current conventional methods for its management, there is a call for discovering advanced pest management approaches. RNA interference (RNAi) is an emerging molecular tool showing flexible potential for the management of . We conducted a search of the recent application of RNAi literature using Google Scholar and Mendeley to find advanced papers on management using RNAi molecular tools that led to growth inhibition, developmental aberrations, reduced fecundity, and mortality, mainly by disruption of normal biological processes of the pest. Although efforts have been made to accelerate the utility of RNAi, many factors limit the efficiency of RNAi to achieve successful control over . Owing to RNAi's potential bioactivity and economic and ecological acceptability, continued research efforts should focus on improving its broad applicability, including field conditions. Screening and identification of key target genes should be a priority task to achieve effective and sustainable management of this insect via RNAi. In addition, a clear understanding of the present status of RNAi utilization in management is of paramount importance to improve its efficiency. Therefore, in this review, we highlight the biology of and the RNAi mechanism as a foundation for the molecular management of the pest. Then, we discuss the current knowledge of the RNAi approach in management and the factors affecting the efficiency of RNAi application. Finally, the prospects for RNAi-based insect pest management are highlighted for future research to achieve effective management of
草地贪夜蛾（鳞翅目：夜蛾科）是一种入侵性害虫，会损害玉米和高粱，而玉米和高粱是包括非洲在内的新殖民农业生态系统中的重点作物。由于该害虫的侵染不断增加，以及当前常规防治方法存在局限性，人们呼吁探索先进的害虫管理方法。RNA干扰（RNAi）是一种新兴的分子工具，在害虫管理方面显示出灵活的潜力。我们使用谷歌学术和 Mendeley 搜索了近期关于RNAi文献的应用，以查找使用RNAi分子工具进行害虫管理的高级论文，这些工具主要通过破坏害虫的正常生物过程，导致其生长抑制、发育异常、繁殖力下降和死亡。尽管已经努力加速RNAi的应用，但许多因素限制了RNAi成功控制害虫的效率。由于RNAi具有潜在的生物活性以及经济和生态可接受性，持续的研究工作应集中在提高其广泛适用性，包括田间条件。筛选和鉴定关键靶基因应是通过RNAi实现对这种昆虫有效和可持续管理的首要任务。此外，清楚了解RNAi在害虫管理中的应用现状对于提高其效率至关重要。因此，在本综述中，我们强调了害虫的生物学特性和RNAi机制，作为害虫分子管理的基础。然后，我们讨论了RNAi方法在害虫管理中的当前知识以及影响RNAi应用效率的因素。最后，强调了基于RNAi的害虫管理的前景，以供未来研究实现对……的有效管理
