State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Immunol. 2022 Sep 8;13:969517. doi: 10.3389/fimmu.2022.969517. eCollection 2022.
SERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To date, there have been no reports on the immune function of SERPINA1 in fishes. In this study, we first cloned the gene of grass carp () and found that it could respond rapidly to the infection of Grass carp reovirus (GCRV), and overexpression of could enhance the antiviral response of CIK cells. A polyclonal antibody of SERPINA1 was prepared, and the protein interacting with SERPINA1 was screened by CoIP/MS in grass carp hepatopancreas tissue. It was found that SERPINA1 interacted with coagulation factor 2 (CF2) and could degrade it in a dose-dependent manner. In addition, overexpression of contributed to the infection of GCRV in CIK cells, whereas co-expression of and in grass carp reduced the copy number of GCRV in cells. The results showed that grass carp SERPINA1 could inhibit GCRV infection by degrading CF2. This study proposes that SERPINA1 can inhibit viral infection through interaction with the coagulation factor, providing new insights into the molecular mechanism of SERPINA1's antiviral function.
丝氨酸蛋白酶抑制剂家族 1 成员（SERPINA1）通过调节丝氨酸和半胱氨酸蛋白酶的活性在病毒感染和炎症中发挥作用。迄今为止，尚未有关于鱼类 SERPINA1 免疫功能的报道。在本研究中，我们首先克隆了草鱼（）基因，并发现它可以对草鱼出血病病毒（GCRV）的感染迅速作出反应，并且过表达可以增强 CIK 细胞的抗病毒反应。制备了 SERPINA1 的多克隆抗体，并通过 CoIP/MS 在草鱼肝胰腺组织中筛选与 SERPINA1 相互作用的蛋白。结果发现 SERPINA1 与凝血因子 2（CF2）相互作用，并可以以剂量依赖的方式降解它。此外，过表达在 CIK 细胞中有助于 GCRV 的感染，而在草鱼中共同表达和则降低了细胞中 GCRV 的拷贝数。结果表明，草鱼 SERPINA1 可以通过降解 CF2 来抑制 GCRV 感染。本研究提出 SERPINA1 可以通过与凝血因子相互作用来抑制病毒感染，为 SERPINA1 的抗病毒功能的分子机制提供了新的见解。
