Ren Yi, Zhang Xiaoxia, He Fang, Bao Ting, Wang Ting, Li Yiwei, Wang Rui, Wang Hao, Yang Shaoqi
Clinical Medical College, Department of Gastroenterology, General Hospital, Ningxia Medical University, Yinchuan 750004, China.
College of Chinese Traditional Medicine, Ningxia Medical University, Yinchuan 750004, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Oct;38(10):865-871.
Objective To investigate the effect of butyrate on monocytic myeloid-derived suppressor cells (M-MDSCs) and plasma inflammatory cytokines in mice with alcoholic liver disease (ALD). Methods Sixty female C57BL/6J mice were randomly divided into 4 groups: negative control group, ALD model group, sodium butyrate (NaB)-treated control group and NaB-treated ALD model group. The control groups were fed with ethanol-free modified Lieber-DeCarli liquid diets, while the model groups were fed with ethanol-containing modified Lieber-DeCarli liquid diets. Meanwhile, mice in the NaB-treated groups were given a liquid diet containing NaB (0.6 g/kg). After 6 weeks of feeding, the proportions of M-MDSCs in the peripheral blood, liver and spleen cells were detected by flow cytometry. Concentrations of plasma inflammatory cytokines including tumor necrosis factor-α (TNF-α) and interleukin -10 (IL-10) were determined by cytometric bead array (CBA). The correlation between the proportion of M-MDSCs and plasma inflammatory cytokines were analyzed. Results Compared with negative control group, the proportions of M-MDSCs of the peripheral blood, liver and spleen cells in ALD model group increased significantly; plasma TNF-α level also dramatically increased; plasma IL-10 concentration, however, decreased considerably. In contrast, compared to ALD model group, the proportions of M-MDSCs of the peripheral blood, liver and spleen cells in NaB-treated ALD model group significantly increased. The level of plasma TNF-α decreased and plasam IL-10 concentration increased after NaB treatment. Moreover, the proportions of M-MDSCs in peripheral blood, liver and spleen were negatively correlated with TNF-α and positively correlated with IL-10. Conclusion NaB may alleviate the progression of ALD by involving M-MDSCs in the peripheral blood, liver, spleen and suppressing inflammation in mice.
目的 探讨丁酸盐对酒精性肝病（ALD）小鼠单核细胞来源的髓系抑制细胞（M-MDSCs）及血浆炎症细胞因子的影响。方法 将60只雌性C57BL/6J小鼠随机分为4组：阴性对照组、ALD模型组、丁酸钠（NaB）处理对照组和NaB处理ALD模型组。对照组给予不含乙醇的改良Lieber-DeCarli液体饲料，而模型组给予含乙醇的改良Lieber-DeCarli液体饲料。同时，NaB处理组的小鼠给予含NaB（0.6 g/kg）的液体饲料。喂养6周后，通过流式细胞术检测外周血、肝脏和脾脏细胞中M-MDSCs的比例。采用细胞计数珠阵列（CBA）测定血浆炎症细胞因子包括肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的浓度。分析M-MDSCs比例与血浆炎症细胞因子之间的相关性。结果 与阴性对照组相比，ALD模型组外周血、肝脏和脾脏细胞中M-MDSCs的比例显著增加；血浆TNF-α水平也显著升高；然而，血浆IL-10浓度显著降低。相比之下，与ALD模型组相比，NaB处理的ALD模型组外周血、肝脏和脾脏细胞中M-MDSCs的比例显著增加。NaB处理后血浆TNF-α水平降低，血浆IL-10浓度升高。此外，外周血、肝脏和脾脏中M-MDSCs的比例与TNF-α呈负相关，与IL-10呈正相关。结论 NaB可能通过参与外周血、肝脏、脾脏中的M-MDSCs并抑制小鼠炎症来减轻ALD的进展。
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