Bao Ting, Wang Zhen, Zhu Lili, Lu Haixia, Wang Ting, Zhang Yanting, Zhang Xiaoxia, Wang Hao, Yang Shaoqi
Clinical Medical College, Department of Gastroenterology, General Hospital, Ningxia Medical University, Yinchuan 750004, China.
Clinical Medical College, Ningxia Medical University, Yinchuan 750004, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Mar;36(3):228-235.
Objective To investigate the effect of inulin on monocytic myeloid-derived suppressor cells (M-MDSCs) and plasma inflammatory cytokines in mouse models with non-alcoholic fatty liver disease (NAFLD). Methods C57BL/6 mice were randomly divided into 4 groups: normal diet group, inulin-treated control group, NAFLD model group and inulin-treated NAFLD model group. The normal diet group and inulin-treated control group were given normal diet daily, while the other two groups were fed high-fat diet (60% fat) instead. Meanwhile, the mice in the two inulin-treated groups were administrated dietary inulin (5 g/kg). After 14 weeks of feeding, the mice were sacrificed and their peripheral blood, liver and spleen cells were collected. The proportion of M-MDSCs in the peripheral blood, spleen and liver cells were separately detected by flow cytometry. Concentrations of plasma inflammatory cytokines including tumor necrosis factor α (TNF-α) and interleukin 10 (IL-10) were determined by flow cytometry CBA. Statistical Pearson was used to analyze the correlation between the proportion of M-MDSCs and plasma inflammatory cytokines. Results Compared with the normal diet group, the proportion of M-MDSCs in the peripheral blood, liver and spleen of the NAFLD model group significantly increased, plasma TNF-α level also dramatically increased, but IL-10 concentration showed no significant difference from that of the normal diet group. Conversely, INU treatment, compared with the NAFLD model group without INU treatment, increased the proportion of M-MDSCs in the peripheral blood, liver and spleen notably, decreased plasma TNF-α level, and increased plasma IL-10 concentration. The proportion of M-MDSCs in the peripheral blood, liver and spleen cells of NAFLD mice were negatively correlated with plasma TNF-α level, but positively correlated with plasma IL-10 concentration. Conclusion INU may alleviate the progression of NAFLD via recruiting M-MDSCs in the peripheral blood, liver and spleen and reducing inflammation in mice.
目的 探讨菊粉对非酒精性脂肪性肝病（NAFLD）小鼠模型中单核细胞来源的髓系抑制细胞（M-MDSCs）及血浆炎性细胞因子的影响。方法 将C57BL/6小鼠随机分为4组：正常饮食组、菊粉处理对照组、NAFLD模型组和菊粉处理NAFLD模型组。正常饮食组和菊粉处理对照组每日给予正常饮食，而另外两组则改为高脂饮食（脂肪含量60%）。同时，两个菊粉处理组的小鼠给予膳食菊粉（5 g/kg）。喂养14周后，处死小鼠并收集其外周血、肝脏和脾脏细胞。通过流式细胞术分别检测外周血、脾脏和肝脏细胞中M-MDSCs的比例。采用流式细胞术CBA法测定血浆炎性细胞因子包括肿瘤坏死因子α（TNF-α）和白细胞介素10（IL-10）的浓度。采用Pearson统计学方法分析M-MDSCs比例与血浆炎性细胞因子之间的相关性。结果 与正常饮食组相比，NAFLD模型组外周血、肝脏和脾脏中M-MDSCs的比例显著增加，血浆TNF-α水平也显著升高，但IL-10浓度与正常饮食组无显著差异。相反，与未用菊粉处理的NAFLD模型组相比，菊粉处理显著增加了外周血、肝脏和脾脏中M-MDSCs的比例，降低了血浆TNF-α水平，并增加了血浆IL-10浓度。NAFLD小鼠外周血、肝脏和脾脏细胞中M-MDSCs的比例与血浆TNF-α水平呈负相关，但与血浆IL-10浓度呈正相关。结论 菊粉可能通过募集外周血、肝脏和脾脏中的M-MDSCs并减轻小鼠炎症来缓解NAFLD的进展。
