circZC3HAV1 通过调控 TBC1D9 影响结直肠癌细胞的生物学行为。
circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells.
机构信息
Department of Gastrointestinal Surgery, The Second People's Hospital of Liaocheng, Liaocheng, 252600 Shandong, China.
Department of Gastroenterology, The Second People's Hospital of Liaocheng, Liaocheng, 252600 Shandong, China.
出版信息
Biomed Res Int. 2022 Sep 16;2022:7386946. doi: 10.1155/2022/7386946. eCollection 2022.
BACKGROUND
Colorectal cancer (CRC) is one of the most frequently diagnosed cancers all over the world, which accounts for a large proportion of cancer-associated deaths. The regulatory function of circular RNAs (circRNAs) has been affirmed in diverse cancers. circ_0082628, named circRNA zinc finger CCCH-type containing antiviral 1 (circZC3HAV1), has been discovered to be significantly downregulated in CRC tissues. Nevertheless, the function and mechanism of circZC3HAV1 in CRC remain unclear.
PURPOSE
We targeted at studying the specific role and mechanism of circZC3HAV1 in CRC cells.
METHODS
The expression of the genes was detected by quantitative real-time polymerase chain reaction (qPCR). The binding relationship among different genes was verified by mechanism assays. Functional assays were carried out to reveal the role of different RNAs in CRC cell malignant behaviors.
RESULTS
circZC3HAV1 was significantly downregulated in CRC cells. circZC3HAV1 overexpression hampered CRC cell migratory and invasive abilities. As for the mechanism, circZC3HAV1 competitively bound with microRNA-146b-3p (miR-146b-3p) to enhance the expression of TBC1 domain family member 9 (TBC1D9). Rescue assays demonstrated circZC3HAV1 sponged miR-146b-3p and upregulated TBC1D9 to restrict migration and invasion of CRC cells.
CONCLUSION
circZC3HAV1 could upregulate TBC1D9 via absorbing miR-146b-3p, consequently inhibiting migratory and invasive capabilities of CRC cells.
背景
结直肠癌（CRC）是全球最常见的癌症之一，占癌症相关死亡的很大比例。环状 RNA（circRNAs）的调控功能已在多种癌症中得到证实。circ_0082628，命名为环状 RNA 锌指 CCCH 型含有抗病毒 1（circZC3HAV1），已被发现在 CRC 组织中显著下调。然而，circZC3HAV1 在 CRC 中的功能和机制仍不清楚。
目的
我们旨在研究 circZC3HAV1 在 CRC 细胞中的具体作用和机制。
方法
通过定量实时聚合酶链反应（qPCR）检测基因的表达。通过机制分析验证不同基因之间的结合关系。进行功能分析以揭示不同 RNA 在 CRC 细胞恶性行为中的作用。
结果
circZC3HAV1 在 CRC 细胞中显著下调。circZC3HAV1 的过表达抑制了 CRC 细胞的迁移和侵袭能力。至于机制，circZC3HAV1 竞争性结合 microRNA-146b-3p（miR-146b-3p）以增强 TBC1 结构域家族成员 9（TBC1D9）的表达。挽救实验表明，circZC3HAV1 吸附 miR-146b-3p 并上调 TBC1D9 以限制 CRC 细胞的迁移和侵袭。
结论
circZC3HAV1 可通过吸收 miR-146b-3p 上调 TBC1D9，从而抑制 CRC 细胞的迁移和侵袭能力。
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