Lee S D, Wang J Y, Wu J C, Tsai Y T, Lo K J, Lai K H, Tsay S H, Govindarajan S
Scand J Infect Dis. 1987;19(2):173-7. doi: 10.3109/00365548709032395.
In 86 Chinese patients with histologically proven hepatitis B surface antigen (HBsAg) positive chronic hepatitis and serum alanine aminotransferase levels exceeding 200 U/l, antibody to hepatitis D antigen (HDAg) was detected more frequently in sera from hepatitis B e antigen (HBeAg) negative patients (11/35, 31.4%) than in HBeAg positive (4/51, 7.8%) patients (p less than 0.02). 10 liver biopsy specimens (76.9%) from 13 chronic hepatitis B patients with superimposed hepatitis D virus (HDV) infection, showed positive staining for HDAg in their hepatocytes. Neither HBsAg nor hepatitis B core antigen (HBcAg) was found in the liver in 12/13 patients with superimposed HDV infection. However, in liver biopsy specimens from 42 patients without HDV superinfection, HBsAg was stained positively in 41 patients (97.6%), and HBcAg in 24 patients (47.1%). Using dot blot hybridization technique, serum hepatitis B virus (HBV) DNA was detected in 62.1% (41/66) of patients without HDV superinfection, while it was detected only in 10.0% (1/10) of patients who had HDV superinfection. It is concluded that HDV superinfection plays a significant role in Taiwan in HBeAg negative chronic hepatitis B patients with clinical "exacerbation". The data show clear evidence of HDV interfering with the replication of HBV.
在86例经组织学证实为乙型肝炎表面抗原（HBsAg）阳性的慢性肝炎且血清丙氨酸转氨酶水平超过200 U/l的中国患者中，乙型肝炎e抗原（HBeAg）阴性患者血清中丁型肝炎抗原抗体（抗-HDAg）的检出率（11/35，31.4%）高于HBeAg阳性患者（4/51，7.8%）（p<0.02）。13例重叠感染丁型肝炎病毒（HDV）的慢性乙型肝炎患者的10份肝活检标本（76.9%），其肝细胞中HDAg染色呈阳性。13例重叠感染HDV的患者中有12例在肝脏中未发现HBsAg和乙型肝炎核心抗原（HBcAg）。然而，在42例未重叠感染HDV的患者的肝活检标本中，41例（97.6%）HBsAg染色呈阳性，24例（47.1%）HBcAg染色呈阳性。采用斑点杂交技术，在未重叠感染HDV的患者中，62.1%（41/66）检测到血清乙型肝炎病毒（HBV）DNA，而在重叠感染HDV的患者中仅10.0%（1/10）检测到。结论是，在台湾，HDV重叠感染在临床“恶化”的HBeAg阴性慢性乙型肝炎患者中起重要作用。数据清楚地表明HDV干扰了HBV的复制。
