Liang S P, Yang D, Zhang L X
Sci Sin B. 1987 Feb;30(2):149-60.
The amino acid sequences of tryptic, chymotryptic and cyanogen bromide cleavage peptides of the lactate dehydrogenase isozyme M4 from giant panda have been determined. Based on the overlapping peptides and by a comparison with the known sequence of porcine lactate dehydrogenase isozyme M subunit, the complete primary structure of the giant panda lactate dehydrogenase isozyme M subunit has been established. The polypeptide chain of giant panda lactate dehydrogenase isozyme M subunit consists of 331 amino acid residues. There is a variance of 17 amino acid residues between the porcine and the giant panda lactate dehydrogenase isozyme M subunits. Most of the variable residues are substitutions by chemically similar amino acids and take place at residues not related to the active center of the enzyme.
大熊猫乳酸脱氢酶同工酶M4经胰蛋白酶、胰凝乳蛋白酶和溴化氰裂解后，其肽段的氨基酸序列已被测定。基于重叠肽段，并与猪乳酸脱氢酶同工酶M亚基的已知序列进行比较，已确定大熊猫乳酸脱氢酶同工酶M亚基的完整一级结构。大熊猫乳酸脱氢酶同工酶M亚基的多肽链由331个氨基酸残基组成。猪和大熊猫乳酸脱氢酶同工酶M亚基之间有17个氨基酸残基不同。大多数可变残基被化学性质相似的氨基酸取代，且发生在与酶活性中心无关的残基处。
