Liu P, Xu H, Lin Y T, Xiao Z M, Liu L, Jiang K
Sci Sin B. 1987 Feb;30(2):169-75.
The principle of the liquid and solid two-phase radioimmunoassay and its application to measuring the concentrations of triiodothyronine and thyroxine of human serum in a single sample at the same time are described in this paper. Rabbit anti-triiodothyronine antiserum was immobilized on the inner surface of a plastic tube as solid-phase radioimmunoassay system of testing triiodothyronine and donkey anti-rabbit gamma globulin antiserum was used to separate immunocomplex in liquid-phase radioimmunoassay system of thyroxine reaction. In both systems, radioiodine-125-labelled antigens were used as tracer. Through simple calculation, we can get the results of serum concentrations of triiodothyronine and thyroxine in a single sample. Compared with classical single-component radioimmunoassay of triiodothyronine and thyroxine and by methodological appraisal of two-phase radioimmunoassay itself, this method has the advantages of very high efficiency, reliability and also has the same specificity and precision.
本文介绍了液固两相放射免疫分析法的原理及其在同时测定单一样本人血清中三碘甲状腺原氨酸和甲状腺素浓度方面的应用。将兔抗三碘甲状腺原氨酸抗血清固定在塑料管内表面作为检测三碘甲状腺原氨酸的固相放射免疫分析系统，用驴抗兔γ球蛋白抗血清在甲状腺素反应的液相放射免疫分析系统中分离免疫复合物。在这两个系统中，均使用放射性碘-125标记的抗原作为示踪剂。通过简单计算，可得出单一样本中血清三碘甲状腺原氨酸和甲状腺素的浓度结果。与经典的三碘甲状腺原氨酸和甲状腺素单组分放射免疫分析相比，通过对两相放射免疫分析本身的方法学评价，该方法具有效率极高、可靠性强的优点，且具有相同的特异性和精密度。
