糖尿病神经病变患者长链非编码 RNA NEAT1/miR-199a-5/BiP 轴的失调。
Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy.
机构信息
Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
出版信息
Lab Med. 2023 Mar 7;54(2):160-165. doi: 10.1093/labmed/lmac082.
OBJECTIVE
Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy.
METHODS
Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression.
RESULTS
The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated.
CONCLUSION
As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis.
目的
糖尿病神经病变（DN）是一种神经损伤，也是糖尿病最常见的并发症。关于内质网（ER）应激与神经病变发病机制的关系，本研究旨在检测 DN 患者与无神经病变糖尿病患者外周血中结合免疫球蛋白蛋白（BiP）基因表达和长非编码 RNA 核富集丰富转录物 1（NEAT1）、作为其调节剂的 miR-199a-5。
方法
从 20 例 DN 患者和 20 例无神经病变糖尿病患者（非-DN）中获得外周血样本。从外周血单核细胞中提取 RNA 后，进行逆转录定量聚合酶链反应以评估 RNA 表达。
结果
结果表明，DN 组的 NEAT1 和 BiP 基因表达水平与非-DN 组相比显著增加。此外，DN 组中 miR-199a-5p 的表达水平显著下调。
结论
因此，NEAT1、miR-199a-5p 和 BiP 的轴可能在 DN 的发病机制中发挥作用。
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