Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Banyuls-sur-Mer, France.
PLoS One. 2022 Sep 27;17(9):e0275193. doi: 10.1371/journal.pone.0275193. eCollection 2022.
Photoconvertible proteins are powerful tools widely used in cellular biology to study cell dynamics and organelles. Over the past decade, photoconvertible proteins have also been used for developmental biology applications to analyze cell lineage and cell fate during embryonic development. One of these photoconvertible proteins called Kaede, from the stony coral Trachyphyllia geoffroyi, undergoes irreversible photoconversion from green to red fluorescence when illuminated with UV light. Undertaking a cell tracing approach using photoconvertible proteins can be challenging when using unconventional animal models. In this protocol, we describe the use of Kaede to track specific cells during embryogenesis of the cephalochordate Branchiostoma lanceolatum. This protocol can be adapted to other unconventional models, especially marine animals.
光转化蛋白是细胞生物学中广泛用于研究细胞动态和细胞器的强大工具。在过去的十年中，光转化蛋白也被用于发育生物学应用，以分析胚胎发育过程中的细胞谱系和细胞命运。其中一种叫做 Kaede 的光转化蛋白来自石珊瑚 Trachyphyllia geoffroyi，当用紫外光照射时，它会从绿色不可逆地转化为红色荧光。当使用非传统的动物模型时，使用光转化蛋白进行细胞追踪可能具有挑战性。在本方案中，我们描述了使用 Kaede 追踪文昌鱼胚胎发生过程中特定细胞的方法。该方案可以适应其他非传统模型，尤其是海洋动物。
