College of Enology, Northwest A&F University, Yangling 712100, China.
J Agric Food Chem. 2022 Oct 12;70(40):12807-12818. doi: 10.1021/acs.jafc.2c03838. Epub 2022 Sep 27.
To study the role of infrared (IR) radiation in the color change of the grape berry, field screening (IR-) and in vitro culture irradiation (IR+) were used. Acylated anthocyanin biosyntheses, including the biosynthesis of malvidin 3--glucoside, peonidin 3--glucoside, and their derivatives (acetylation and -coumaroylation), were inhibited by IR-. IR+ promoted the biosynthesis of malvidin 3--glucoside and its derivatives, and IR+ inhibited the biosynthesis of peonidin 3--glucoside and its derivatives. WGCNA analysis revealed that the red module positively correlated with the flavonoid pathway. The hub genes were related to the anthocyanin pathway, including '', , , , and . Further, the results revealed that transcription factors like RLK-Pelle, MYB, and C2H2 families were involved in response to IR radiation. Therefore, these results provide a complete understanding of IR radiation in grape skin color formation and the prospect of using supplemental light to improve the overall color of berries.
为了研究红外（IR）辐射在葡萄浆果颜色变化中的作用，采用了田间筛选（IR-）和体外培养辐照（IR+）。酰化花色苷生物合成，包括矢车菊素 3-葡萄糖苷、芍药素 3-葡萄糖苷及其衍生物（乙酰化和 -香豆酰化）的生物合成，被 IR-抑制。IR+促进了矢车菊素 3-葡萄糖苷及其衍生物的生物合成，而 IR+抑制了芍药素 3-葡萄糖苷及其衍生物的生物合成。WGCNA 分析显示，红色模块与类黄酮途径呈正相关。枢纽基因与花色苷途径有关，包括、、、、和。此外，结果表明，转录因子如 RLK-Pelle、MYB 和 C2H2 家族参与了对 IR 辐射的反应。因此，这些结果提供了对 IR 辐射在葡萄果皮颜色形成中的完整理解，并为利用补充光照来改善浆果整体颜色提供了前景。
