Favoreto A L, Carvalho V R, Domingues M M, Ribeiro M F, Cavallini G, Lawson S A, Silva W M, Zanuncio J C, Wilcken C F
Universidade Estadual Paulista - UNESP, Faculdade de Ciências Agronômicas, Departamento de Proteção Vegetal, Botucatu, SP, Brasil.
University of the Sunshine Coast, Department of Agriculture, Fisheries and Forestry Queensland, Brisbane, Australia.
Braz J Biol. 2022 Sep 23;82:e264475. doi: 10.1590/1519-6984.264475. eCollection 2022.
The sucking insect, Glycaspis brimblecombei Moore (Hemiptera: Aphalaridae), is originally from Australia and reduces the productivity of Eucalyptus crops. The parasitoid Psyllaephagus bliteus Riek (Hymenoptera: Encyrtidae) is the main agent used in the integrated management of G. brimblecombei. Endosymbionts, in insects, are important in the adaptation and protection of their hosts to the environment. The intracellular symbionts Wolbachia, induces reproductive changes such as cytoplasmic incompatibility, feminization, male death and parthenogenesis. The objective of this study was to report the first record of Wolbachia pipientis in populations of G. brimblecombei and of its parasitoid P. bliteus in the field in Brazil. Branches with adults of G. brimblecombei and P. bliteus were collected from eucalyptus trees in commercial farms in six Brazilian states and, after emergence, the insects obtained were frozen at -20 °C. Polymerase chain reaction (PCR) was performed to detect the Wolbachia endosymbiont. Wolbachia pipientis was identified in individuals of G. brimblecombei and its parasitoid P. bliteus from populations of the counties of Agudos and Mogi-Guaçu (São Paulo State), Itamarandiba (Minas Gerais State) and São Jerônimo da Serra (Paraná State) in Brazil.
吸食性昆虫，布里姆比氏桉木虱（Glycaspis brimblecombei Moore，半翅目：澳木虱科）原产于澳大利亚，会降低桉树林的生产力。寄生蜂布莱特氏木虱啮小蜂（Psyllaephagus bliteus Riek，膜翅目：跳小蜂科）是用于综合防治布里姆比氏桉木虱的主要媒介。昆虫体内的共生菌对于宿主适应和保护自身免受环境影响十分重要。细胞内共生菌沃尔巴克氏体可诱导诸如细胞质不亲和、雌性化、雄性死亡和孤雌生殖等生殖变化。本研究的目的是报告在巴西野外的布里姆比氏桉木虱种群及其寄生蜂布莱特氏木虱啮小蜂种群中首次发现的嗜皮菌沃尔巴克氏体。从巴西六个州的商业农场的桉树上采集带有布里姆比氏桉木虱成虫和布莱特氏木虱啮小蜂成虫的树枝，待昆虫羽化后，将获得的昆虫在-20℃下冷冻。进行聚合酶链反应（PCR）以检测沃尔巴克氏体共生菌。在巴西圣保罗州阿古多斯县和莫吉-瓜苏县、米纳斯吉拉斯州伊塔马兰迪巴县以及巴拉那州圣热罗尼莫-达塞拉县的布里姆比氏桉木虱及其寄生蜂布莱特氏木虱啮小蜂个体中鉴定出了嗜皮菌沃尔巴克氏体。
