Favoreto Ana Laura, Domingues Maurício Magalhães, de Carvalho Vanessa Rafaela, Ribeiro Murilo Fonseca, Zanuncio José Cola, Wilcken Carlos Frederico
Departamento de Proteção Vegetal, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista (UNESP), Avenida Universitária, 3780, Botucatu, São Paulo, 18610-034, Brasil.
Departamento de Entomologia/BIOAGRO, Universidade Federal de Viçosa, José Cola Zanuncio, Viçosa, 36570-900, Minas Gerais, Brasil.
Braz J Microbiol. 2024 Dec;55(4):3075-3079. doi: 10.1007/s42770-024-01465-0. Epub 2024 Jul 23.
Eucalyptus is the most intensively managed tree genus in the world. Different factors, including damage by insect pests, affect its growth and productivity. Among these pests is Glycaspis brimblecombei Moore (Hemiptera: Aphalaridae), an exotic insect of Australian origin. The evolutionary success of this insect depends on symbiotic associations with microorganisms. The influence of these microorganisms on insect pests and their natural enemies is important for integrated management tactics. Within this context, this work aimed to detect Arsenophonus in populations of G. brimblecombei in Brazil. Eucalyptus branches infested with G. brimblecombei nymphs were collected in commercial eucalyptus plantations in six Brazilian states. Specimens of this pest were sampled soon after emergence and frozen for molecular analysis. The genomic DNA of G. brimblecombei adults from each population was extracted and used to detect the endosymbiont Arsenophonus by polymerase chain reaction (PCR) employing specific primers that target its 23 S rRNA gene. This endosymbiont was identified in all of the studied G. brimblecombei populations. This is the first report on the association between Arsenophonus and G. brimblecombei in Brazil.
桉树是世界上管理最为集约的树木属。包括害虫危害在内的不同因素会影响其生长和生产力。这些害虫中有来自澳大利亚的外来昆虫——布林布尔考姆桉小卷蛾（半翅目：斑蚜科）。这种昆虫的进化成功依赖于与微生物的共生关系。这些微生物对害虫及其天敌的影响对于综合管理策略至关重要。在此背景下，本研究旨在检测巴西布林布尔考姆桉小卷蛾种群中的嗜菌属。在巴西六个州的商业桉树林中收集了被布林布尔考姆桉小卷蛾若虫侵染的桉树枝条。这种害虫的标本在羽化后不久进行采样并冷冻，用于分子分析。提取每个种群中布林布尔考姆桉小卷蛾成虫的基因组DNA，并使用针对其23S rRNA基因的特异性引物，通过聚合酶链反应（PCR）检测内共生菌嗜菌属。在所有研究的布林布尔考姆桉小卷蛾种群中都鉴定出了这种内共生菌。这是巴西关于嗜菌属与布林布尔考姆桉小卷蛾之间关联的首次报道。
