Laboratory of Experimental Pathophysiology, Department of Biological Sciences and Centre of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, Brazil.
Laboratory of Immunobiology of Inflammation, Department of Biological Sciences, Institute of Physics and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, Brazil.
Life Sci. 2022 Nov 15;309:121004. doi: 10.1016/j.lfs.2022.121004. Epub 2022 Sep 25.
In this study, the effects of exposure to isoflurane, sevoflurane and desflurane on the oxidative response and inflammation at different times was analyzed in the lungs of adult C57BL/6 mice. 120 animals were divided into 3 groups (n = 40): Isoflurane (ISO), Sevoflurane (SEV) and Desflurane (DES) and exposed to these anesthetics for 1 h (n = 10), 2 h (n = 10) and 3 h (n = 10), at a minimum alveolar concentration (MAC) equal to 1. The control group (CG) (n = 10) was exposed to ambient air. 24 h after the experimental protocol, the animals were euthanized and the bronchoalveolar lavage fluid (BALF), blood and lung tissue samples were collected. In the BALF, animals exposed to isoflurane for 2 h and 3 h showed a greater influx of leukocytes, especially macrophages compared to the CG. The ISO3h had lower leukocyte counts in the peripheral blood compared to CG, ISO1h and ISO2h. There was an increase in CCL-2 levels in the ISO3h compared to the CG. Superoxide dismutase activity was higher in ISO1h compared to CG. The activity of catalase was higher in the ISO1h and ISO2h compared to the CG. The lipid peroxidation, as well as carbonylated protein were higher in the ISO3h compared to the CG (p < 0.05). Similar results were observed in the exposure of SEV and DES compared to inflammation and redox imbalance in different periods. This study demonstrated that time is a determinant to promote a local and systemic inflammatory response to different inhalational anesthetics in a healthy murine model.
在这项研究中，分析了暴露于异氟烷、七氟烷和地氟烷对成年 C57BL/6 小鼠肺部氧化反应和炎症的不同时间影响。将 120 只动物分为 3 组（n=40）：异氟烷（ISO）、七氟烷（SEV）和地氟烷（DES），并以最低肺泡浓度（MAC）等于 1 分别暴露于这些麻醉剂 1 小时（n=10）、2 小时（n=10）和 3 小时（n=10）。对照组（CG）（n=10）暴露于环境空气中。实验方案 24 小时后，处死动物并收集支气管肺泡灌洗液（BALF）、血液和肺组织样本。在 BALF 中，与 CG 相比，暴露于异氟烷 2 小时和 3 小时的动物白细胞，特别是巨噬细胞的流入量更大。与 CG 相比，ISO3h 在外周血中的白细胞计数更低。与 CG 相比，ISO3h 中的 CCL-2 水平增加。与 CG 相比，ISO1h 中的超氧化物歧化酶活性更高。与 CG 相比，ISO1h 和 ISO2h 中的过氧化氢酶活性更高。与 CG 相比，ISO3h 中的脂质过氧化以及羰基化蛋白更高（p<0.05）。与炎症和不同时期的氧化还原失衡相比，在 SEV 和 DES 的暴露中也观察到了类似的结果。本研究表明，时间是在健康小鼠模型中促进不同吸入麻醉剂引起局部和全身炎症反应的决定因素。
