Zhen Hui, Zheng Mingyue, Geng Huazhi, Song Qian, Gao Lili, Yuan Zuoqing, Deng Hongkuan, Pang Qiuxiang, Zhao Bosheng
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Nantong, China.
Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
Front Mol Neurosci. 2022 Sep 12;15:988803. doi: 10.3389/fnmol.2022.988803. eCollection 2022.
Disturbances in the excitatory/inhibitory balance of brain neural circuits are the main source of encephalopathy during neurodevelopment. Changes in the function of neural circuits can lead to depolarization or repeat rhythmic firing of neurons in a manner similar to epilepsy. GABAergic neurons are inhibitory neurons found in all the main domains of the CNS. Previous studies suggested that and play a crucial role in the regulation of GABAergic neurons during planarian regeneration. However, the mechanisms behind the regeneration of GABAergic neurons have not been fully explained. Herein, we demonstrated that DjCamkII and DjCaln were mutual negative regulation during planarian head regeneration. exerted feedback positive regulation on both and . Whole-mount hybridization (WISH) and fluorescence hybridization (FISH) revealed that was predominantly expressed in the pharynx and parenchymal cells in intact planarian. Interestingly, during planarian head regeneration, was predominantly located in the newborn brain. Down-regulation of led to regeneration defects in the brain including regenerative brain became small and the lateral nerves cannot be regenerated completely, and a decreasein the number of GABAergic neurons during planarian head regeneration. These findings suggest that the feedback loop between , and is crucial for the formation of GABAergic neurons during planarian head regeneration.
脑神经网络兴奋性/抑制性平衡的紊乱是神经发育过程中脑病的主要根源。神经回路功能的变化可导致神经元去极化或以类似于癫痫的方式重复节律性放电。GABA能神经元是中枢神经系统所有主要区域中发现的抑制性神经元。先前的研究表明，[具体物质1]和[具体物质2]在涡虫再生过程中对GABA能神经元的调节中起关键作用。然而，GABA能神经元再生背后的机制尚未得到充分解释。在此，我们证明了DjCamkII和DjCaln在涡虫头部再生过程中相互负调控。[具体物质3]对[具体物质1]和[具体物质2]都发挥反馈正调控作用。全组织原位杂交（WISH）和荧光原位杂交（FISH）显示，[具体物质1]在完整涡虫的咽部和实质细胞中主要表达。有趣的是，在涡虫头部再生过程中，[具体物质1]主要位于新生脑中。[具体物质1]的下调导致脑部再生缺陷，包括再生脑变小且侧神经不能完全再生，以及涡虫头部再生过程中GABA能神经元数量减少。这些发现表明，[具体物质1]、[具体物质2]和[具体物质3]之间的反馈回路对涡虫头部再生过程中GABA能神经元的形成至关重要。
