与人类内皮细胞单层共培养的多细胞膀胱肿瘤球体。

Multicellular bladder tumor spheroids in coculture with human endothelial cell monolayers.

作者信息

Knüchel R, Hofstädter F, Feichtinger J, Recktenwald A, Franke R P, Hollweg H, Rübben H, Rammal E, Jakse G

出版信息

Urol Int. 1987;42(3):176-80. doi: 10.1159/000281890.

Abstract

Multicellular tumor spheroids (MCTS) are avascular spherical tumors grown systematically out of single cells in vitro. The coculture of MCTS of the human bladder carcinoma cell line J 82 with confluent human endothelial cell layers serves as a model to study cell-cell interactions as an important feature in hematogenous tumor spread. Basic information is obtained by phase contrast microscopy and histochemistry. More detailed information is given by the application of scanning and transmission electron microscopy. Immunohistochemistry helps to differentiate endothelial cells from tumor cells (factor VIII-related antigen). The application of this coculture model and of the various methods of evaluation enables us to study cell-to-cell contact phenomena systematically.

摘要

多细胞肿瘤球体（MCTS）是在体外由单细胞系统生长而成的无血管球形肿瘤。人膀胱癌细胞系J82的MCTS与汇合的人内皮细胞层共培养，作为研究细胞间相互作用的模型，细胞间相互作用是血行性肿瘤扩散的一个重要特征。通过相差显微镜和组织化学可获得基本信息。扫描电子显微镜和透射电子显微镜的应用可提供更详细的信息。免疫组织化学有助于区分内皮细胞和肿瘤细胞（VIII因子相关抗原）。这种共培养模型和各种评估方法的应用使我们能够系统地研究细胞间接触现象。