使用分级抗原通过酶联免疫吸附测定（ELISA）对猪囊尾蚴病进行血清学诊断。

Serodiagnosis of porcine cysticercosis by enzyme-linked immunosorbent assay (ELISA) using fractionated antigens.

作者信息

Kumar D, Gaur S N

出版信息

Vet Parasitol. 1987 May;24(3-4):195-202. doi: 10.1016/0304-4017(87)90040-9.

Abstract

The sensitivity and specificity of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Taenia solium cysticercosis was evaluated in experimentally and naturally infected pigs, using T. solium larval scoleces and its fractionated 1st and 2nd peaks on Sephadex G-200 as antigens. First peak antigen gave maximum sensitivity and highest antibody titres. The overall sensitivity of this test was found to be 91.5, 95.8 and 70.8% with scolex, 1st and 2nd peak antigens, respectively. False positive reactions occurred in 9.09% of uninfected pigs with scolex and 1st peak antigens and cross-reactions occurred in 25% of Taenia hydatigena-infected animals using scolex and 2nd peak antigens. No cross-reaction was observed using 1st peak antigen. The specificity of the test was 92.3, 96.2 and 92.3% with scolex, 1st and 2nd peak antigens, respectively.

摘要

采用猪带绦虫幼虫头节及其在Sephadex G - 200上分离的第一和第二峰组分作为抗原，在实验感染和自然感染的猪中评估了酶联免疫吸附测定（ELISA）诊断猪囊尾蚴病的敏感性和特异性。第一峰抗原具有最高的敏感性和最高的抗体滴度。结果发现，该试验使用头节、第一峰和第二峰抗原时的总体敏感性分别为91.5%、95.8%和70.8%。使用头节和第一峰抗原时，9.09%的未感染猪出现假阳性反应；使用头节和第二峰抗原时，25%的感染有泡状带绦虫的动物出现交叉反应。使用第一峰抗原未观察到交叉反应。该试验使用头节、第一峰和第二峰抗原时的特异性分别为92.3%、96.2%和92.3%。