State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Biosens Bioelectron. 2022 Dec 1;217:114726. doi: 10.1016/j.bios.2022.114726. Epub 2022 Sep 22.
Place cells establish rapid mapping relationships between the external environment and themselves in a new context. However, the mapping relationships of environmental cues to place cells in short-term memory is still completely unknown. In this work, we designed a silicon-based motion microelectrode array (mMEA) and an implantation device to record electrophysiological signals of place cells in CA1, CA3, and DG regions in the hippocampus of ten mice in motion, and investigated the corresponding place fields under distal or local cues in just a few minutes. The mMEA can expand the detection area and greatly lower the motion noise. Finding and recording place cells of moving mice in short-term memory is made possible by the mMEA. The place-related cells were found for the first time. Unlike place cells, which only fire in a particular position of the environment, place-related cells fire in numerous areas of the environment. Furthermore, place cells in the CA1 and CA3 have the most stable place memory for time-preferred single cues, and they fire in concert with place-related cells during short-term memory dynamics, whereas place cells in the DG regions have overlapping and unstable place memory in a multi-cue context. These results demonstrate the consistency of place cells in CA1 and CA3 and reflect their different roles in spatial memory processing during familiarization with new environments. The mMEA provides a platform for studying the place cells of short-term memory.
位置细胞在新环境中迅速建立外部环境与其自身之间的映射关系。然而，环境线索与短期记忆中的位置细胞之间的映射关系仍然完全未知。在这项工作中，我们设计了一种基于硅的运动微电极阵列（mMEA）和植入设备，以记录运动中十只小鼠海马 CA1、CA3 和 DG 区位置细胞的电生理信号，并在短短几分钟内研究在远距离或局部线索下的相应位置场。mMEA 可以扩大检测区域，并大大降低运动噪声。mMEA 使得在短期记忆中寻找和记录运动小鼠的位置细胞成为可能。首次发现与位置相关的细胞。与仅在环境的特定位置发射的位置细胞不同，与位置相关的细胞在环境的许多区域发射。此外，CA1 和 CA3 中的位置细胞对时间优先的单个线索具有最稳定的位置记忆，并且在短期记忆动态期间与与位置相关的细胞协同发射，而 DG 区域中的位置细胞在多线索环境中具有重叠且不稳定的位置记忆。这些结果表明 CA1 和 CA3 中的位置细胞具有一致性，并反映了它们在熟悉新环境时处理空间记忆的不同作用。mMEA 为研究短期记忆中的位置细胞提供了一个平台。
