Department of Pathology, University of Health Sciences Turkey, Haseki Training and Research Hospital, ISTANBUL, TURKEY.
Turk Patoloji Derg. 2023;39(2):147-153. doi: 10.5146/tjpath.2022.01590.
Sarcomatoid carcinomas of the lung are a group of aggressive tumors. It has been reported that losses of SMARCA4 and SMARCA2, which play a role in the repair and remodeling of chromatin, contribute to the initiation, progression, and differentiation of neoplasms. The aim of our study was to examine SMARCA4 and SMARCA2 profiles in sarcomatoid carcinomas of the lung.
We screened pleomorphic carcinomas (PCs), carcinosarcomas (CSs), and pulmonary blastomas (PBs). The loss of SMARCA4 and SMARCA2 expression in the tumors was evaluated using immunohistochemical methods. The tumors were also examined to determine immunophenotype, histological tumor diagnosis, surgical resection, tumor histological component, largest tumor diameter, and lymph node metastasis status.
Sixty-nine cases were screened, of which 84% were PCs, 13% were CSs, and 2.8% were PBs. In PCs components, 84.4% were biphasic and 15.5% were monophasic. The PCs showed the most frequent loss of SMARCA4 (25.8%) and SMARCA2 (44.8%). A loss of SMARCA4 and SMARCA2, respectively, was detected in 14.2% and 24.4% in both components of biphasic PCs; 12.2% and 14.2% in the sarcoma component of biphasic PCs; 0% and 8.1% in the carcinoma component of biphasic PCs; 22.2% and 33.3% in monophasic PCs; 0% and 22.2% in both components of CSs; and 0% and 22.2% in the sarcoma component of CSs.
These findings demonstrate a loss of expression of SMARCA4 and SMARCA2 in pulmonary sarcomatoid carcinomas. Loss of the SMARCA complex may be caused by the heterogeneous morphological profile of sarcomatoid carcinomas, independent of tumor histopathological parameters.
肺肉瘤样癌是一组侵袭性肿瘤。据报道，在修复和重塑染色质方面发挥作用的 SMARCA4 和 SMARCA2 的缺失，有助于肿瘤的发生、进展和分化。我们研究的目的是研究肺肉瘤样癌中的 SMARCA4 和 SMARCA2 谱。
我们筛选了多形性癌（PC）、癌肉瘤（CS）和肺胚细胞瘤（PB）。使用免疫组织化学方法评估肿瘤中 SMARCA4 和 SMARCA2 表达的缺失。还检查了肿瘤的免疫表型、组织学肿瘤诊断、手术切除、肿瘤组织学成分、最大肿瘤直径和淋巴结转移状态。
共筛选了 69 例，其中 84%为 PC，13%为 CS，2.8%为 PB。在 PC 成分中，84.4%为双相，15.5%为单相。PC 显示最常见的 SMARCA4（25.8%）和 SMARCA2（44.8%）缺失。双相 PC 的两个成分中分别检测到 SMARCA4 和 SMARCA2 的缺失分别为 14.2%和 24.4%；双相 PC 的肉瘤成分中分别为 12.2%和 14.2%；双相 PC 的癌成分中分别为 0%和 8.1%；单相 PC 中分别为 22.2%和 33.3%；CS 的两个成分中分别为 0%和 22.2%；CS 的肉瘤成分中分别为 0%和 22.2%。
这些发现表明肺肉瘤样癌中存在 SMARCA4 和 SMARCA2 的表达缺失。SMARCA 复合物的缺失可能是由于肉瘤样癌的异质形态学特征，而与肿瘤组织病理学参数无关。
