Using of eosin Y as a facile fluorescence probe in alogliptin estimation: Application to tablet dosage forms and content uniformity testing.

New simple sensitive and reliable spectrofluorimetric approach was established for the determination of the antidiabetic drug; Alogliptin (ALG) in its pure and tablet forms. The developed approach is depended on the suppressive action of ALG on the eosin Y native fluorescence. The quenching action of ALG on the eosin Y native fluorescence was measured at acidic medium pH: 3.5, emission wavelength 541 nm (λ 260 nm). The relative fluorescence intensity (RFI) was measured, and it was directly proportional to ALG concentration in the concentration range of (15-110) μg/mL. The developed and optimized approach was entirely validated regarding to ICH guidelines. The developed method application was successfully extended for ALG content uniformity test (CU). The distribution fraction (DF), rate constants (K), and free energy changes (ΔG°) were calculated. The results obtained were compared to that of the published spectrophotometric one.