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节段和 Churchill1 位置 Notch 配体在 Xenopus 胚层分离过程中的表达。

Nodal and <i>churchill1</i> position the expression of a notch ligand during <i>Xenopus</i> germ layer segregation.

机构信息

Universidad de Buenos Aires, Facultad de Medicina, Departamento de Biología Celular e Histología/1° U.A. Departamento de Histología, Embriología, Biología Celular y Genética, Buenos Aires, Argentina.

CONICET-Universidad de Buenos Aires, Instituto de Biología Celular y Neurociencia "Prof. E. De Robertis" (IBCN), Laboratorio de Embriología Molecular "Prof. Dr. Andrés E. Carrasco", Buenos Aires, Argentina.

出版信息

Life Sci Alliance. 2022 Sep 30;5(12):e202201693. doi: 10.26508/lsa.202201693.

DOI:10.26508/lsa.202201693
PMID:36180230
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9604498/
Abstract

In vertebrates, Nodal signaling plays a major role in endomesoderm induction, but germ layer delimitation is poorly understood. In avian embryos, the neural/mesoderm boundary is controlled by the transcription factor CHURCHILL1, presumably through the repressor ZEB2, but there is scarce knowledge about its role in other vertebrates. During amphibian gastrulation, Delta/Notch signaling refines germ layer boundaries in the marginal zone, but it is unknown the place this pathway occupies in the network comprising Churchill1 and Nodal. Here, we show that <i>Xenopus churchill1</i> is expressed in the presumptive neuroectoderm at mid-blastula transition and during gastrulation, upregulates <i>zeb2</i>, prevents <i>dll1</i> expression in the neuroectoderm, and favors neuroectoderm over endomesoderm development. Nodal signaling prevents <i>dll1</i> expression in the endoderm but induces it in the presumptive mesoderm, from where it activates Notch1 and its target gene <i>hes4</i> in the non-involuting marginal zone. We propose a model where Nodal and Churchill1 position Dll1/Notch1/Hes4 domains in the marginal zone, ensuring the delimitation between mesoderm and neuroectoderm.

摘要

在脊椎动物中，Nodal 信号在中内胚层诱导中起主要作用，但胚层的界限划分知之甚少。在禽类胚胎中，神经/中胚层边界由转录因子 CHURCHILL1 控制，可能通过抑制因子 ZEB2，但在其他脊椎动物中对其作用知之甚少。在两栖动物原肠胚形成过程中，Delta/Notch 信号在边缘区精细划分胚层边界，但在 Churchill1 和 Nodal 组成的网络中，该途径所处的位置尚不清楚。在这里，我们表明 <i>Xenopus churchill1</i> 在中囊胚转换和原肠胚形成过程中表达于神经外胚层前体，上调 <i>zeb2</i>，阻止 <i>dll1</i> 在神经外胚层中的表达，并有利于神经外胚层而不是中内胚层的发育。Nodal 信号阻止 <i>dll1</i> 在内胚层中的表达，但在内胚层前体中诱导其表达，从那里它激活 Notch1 和其靶基因 <i>hes4</i> 在非卷入的边缘区。我们提出了一个模型，其中 Nodal 和 Churchill1 在边缘区定位 Dll1/Notch1/Hes4 结构域，确保中胚层和神经外胚层之间的界限划分。

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