滤泡调节性 T（T）细胞在调节抗体反应中的体内功能分析。

Analysis of the In Vivo Function of Follicular Regulatory T (T) Cells in the Regulation of Antibody Response.

机构信息

School of Pharmacy, Nantong University, Nantong, Jiangsu, China.

Department of Neurosurgery, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.

出版信息

Methods Mol Biol. 2023;2559:3-13. doi: 10.1007/978-1-0716-2647-4_1.

DOI:10.1007/978-1-0716-2647-4_1

PMID:36180622

Abstract

Follicular regulatory T (T) cells, a subset of Foxp3 regulatory T cells (Tregs), play an essential role in the regulation of germinal center (GC) response and humoral self-tolerance. Although it is generally accepted that T cells suppress GC antibody responses mediated by follicular helper T (T) cells and B cells, the helper activity of T cells on GC responses has also been recently reported. Because of this, it is critical to develop specific assays that are able to precisely assess T cell function, particularly its in vivo activity, independent of differentiation and other Tregs. Here we describe an adoptive transfer approach in conjugation with flow cytometry and ELISA to evaluate the T cell function on T, B cells, and antibody response in vivo.

摘要

滤泡调节性 T (T) 细胞是 Foxp3 调节性 T 细胞 (Tregs) 的一个亚群，在调节生发中心 (GC) 反应和体液自身耐受中发挥着重要作用。尽管人们普遍认为 T 细胞抑制滤泡辅助 T (T) 细胞和 B 细胞介导的 GC 抗体反应，但最近也有报道称 T 细胞对 GC 反应具有辅助作用。因此，开发能够精确评估 T 细胞功能（尤其是其体内活性）的特异性检测方法至关重要，而无需考虑分化和其他 Tregs。在这里，我们描述了一种与流式细胞术和 ELISA 相结合的过继转移方法，用于评估 T 细胞在体内对 T 细胞、B 细胞和抗体反应的功能。