用于临床应用的 Treg 制造的 GMP 方案。

A GMP Protocol for the Manufacture of Tregs for Clinical Application.

机构信息

Transplantation Research Immunology Group, Nuffield Department of Surgical Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.

MRC Centre for Transplantation, School of Immunology and Microbial Sciences, King's College London, Guy's Hospital, London, UK.

出版信息

Methods Mol Biol. 2023;2559:205-227. doi: 10.1007/978-1-0716-2647-4_14.

DOI:10.1007/978-1-0716-2647-4_14

PMID:36180635

Abstract

Infusion of regulatory T cells is a promising therapeutic strategy in organ transplantation to modulate the immune system, prevent rejection, minimize the need for pharmaceutical immunosuppression, and improve long-term transplant outcomes. Here we describe a GMP-compliant method we have used for the manufacture of ex vivo expanded autologous regulatory T cells for use in clinical trials.

摘要

输注调节性 T 细胞是器官移植中一种有前途的治疗策略，可调节免疫系统、预防排斥反应、最大限度地减少对药物免疫抑制的需求，并改善长期移植结果。在这里，我们描述了一种符合 GMP 要求的方法，用于制造用于临床试验的体外扩增自体调节性 T 细胞。