Department of Structural and Cellular Biology, Tulane University School of Medicine, Louisiana Cancer Research Consortium, New Orleans, LA, USA.
Methods Mol Biol. 2022;2550:489-496. doi: 10.1007/978-1-0716-2593-4_47.
The tissue-isolated tumor model permits the investigation of melatonin's influence on human tumor growth and metabolism in laboratory rats in vivo. Here we describe a unique surgical technique for implanting and growing human tumor xenografts on a vascular stalk composed of the nude rat epigastric artery and vein that provides a continuous blood supply from a single source to the tissue-isolated tumor while insuring the absence of extraneous vascular connections. A variety of human tumor types may be implanted and grown utilizing this unique model that may provide a plethora of scientific data from a single tumor examined.
组织分离肿瘤模型允许在体内实验大鼠中研究褪黑素对人类肿瘤生长和代谢的影响。在这里，我们描述了一种独特的手术技术，用于在由裸鼠上腹动脉和静脉组成的血管茎上植入和生长人肿瘤异种移植物，该血管茎为组织分离肿瘤提供了来自单一来源的连续血液供应，同时确保不存在外来血管连接。可以利用这种独特的模型植入和生长各种人类肿瘤类型，从单个肿瘤检查中可能提供大量科学数据。
