Jabeen S, Zafar M, Ahmad M, Althobaiti A T, Ozdemir F A, Kutlu M A, Makhkamov T K, Sultana S, Ameen M, Majeed S
Department of Plant Systematics and Biodiversity Lab Quaid-i-Azam University, Islamabad, Pakistan.
Pakistan Academy of Sciences, Islamabad, Pakistan.
Plant Biol (Stuttg). 2023 Jan;25(1):96-106. doi: 10.1111/plb.13473. Epub 2022 Oct 21.
The taxonomic importance of macromorphological and micromorphological seed characteristics was investigated using SEM of ten species of the Salvia genus from Pakistan. The aim was to identify diagnostic seed ultrastructural features that could aid in species delimitation, correct identification and phylogenetic position. The ultrastructure of Salvia varies greatly, and a wide range of unique micromorphological features have been observed. Seed micromorphological features were explored by SEM, including seed shape, colour, texture, cell outline, surface sculpturing, epidermal cell arrangement, anticlinal, and periclinal wall pattern. Seed shapes were categorized as obovate, spherical, spheroid, broadly elliptic, elliptic and oblong, mostly with a terminal hilum. Seed colours were black, light brown, dark brown, brown and yellow. Exo-morphological characters, i.e. epidermal cell arrangements, included irregular, wavy pentagonal-hexagonal, regular pentagonal-hexagonal. Cluster analysis was used to assess similar and distinct species within Salvia with a feasible explanation. Taxonomic keys were made based on micromorphological qualitative features that help to delimit species and identify them quickly within the Salvia genus. Seed morphology of ten Salvia species was described and investigated, and the diagnostic significance of features evaluated using SEM. This study analysed seed features, especially at the species level, which might provide much new taxonomic information. The results revealed that, in seed morphology, using SEM can help with taxon identification, especially at the genus and species levels.
利用扫描电子显微镜对来自巴基斯坦的10种鼠尾草属植物的种子宏观形态和微观形态特征的分类学重要性进行了研究。目的是确定有助于物种界定、正确识别和系统发育位置的诊断性种子超微结构特征。鼠尾草属植物的超微结构差异很大，并且已经观察到了广泛的独特微观形态特征。通过扫描电子显微镜探索了种子的微观形态特征，包括种子形状、颜色、质地、细胞轮廓、表面纹饰、表皮细胞排列、垂周壁和周壁模式。种子形状分为倒卵形、球形、椭球形、宽椭圆形、椭圆形和长方形，大多数带有顶生种脐。种子颜色有黑色、浅棕色、深棕色、棕色和黄色。外部形态特征，即表皮细胞排列，包括不规则、波浪状五边形 - 六边形、规则五边形 - 六边形。采用聚类分析来评估鼠尾草属内相似和不同的物种，并给出合理的解释。基于微观形态定性特征编制了分类检索表，有助于在鼠尾草属内界定物种并快速识别它们。描述并研究了10种鼠尾草属植物的种子形态，并评估了利用扫描电子显微镜观察到的特征的诊断意义。本研究分析了种子特征，特别是在物种水平上，这可能会提供许多新的分类学信息。结果表明，在种子形态方面，使用扫描电子显微镜有助于分类单元的识别，特别是在属和种的水平上。
