Inactivation efficacy and mechanisms of atmospheric cold plasma on : Insight into the influence of growth temperature on survival.

The bactericidal effect of dielectric barrier discharge-atmospheric cold plasma (DBD-ACP, 20, and 30 kV) against on the saline solution and apple juice was investigated. Results show that DBD-ACP is effective for the inactivation of by causing significant changes in cell membrane permeability and bacterial morphology. The effect of culture temperatures on the resistance of to DBD-ACP was also studied. cells grown at 25°C had the lowest resistance but it was gradually increased as the culture temperature was increased (25-45°C) ( < 0.05). Moreover, results from Fourier transform infrared spectroscopy (FT-IR) and Gas Chromatography-Mass Spectrometer (GC-MS) analysis showed that the increase in the culture temperature can gradually cause the decreased level of cyclohexaneundecanoic acid in the cell membrane of ( < 0.05). In contrast, cyclopentaneundecanoic acid, palmitic acid, and stearic acid showed an increasing trend in which the fluidity of the bacterial cell membrane decreased. This study shows a specific correlation between the resistance of and the fatty acid composition of the cell membrane to DBD-ACP.