School of Food Science, Henan institute of Science and Technology, Xinxiang, Henan, 453003, China.
Food Microbiol. 2019 Jun;80:77-84. doi: 10.1016/j.fm.2019.01.003. Epub 2019 Jan 7.
For the purpose of investigating the heat resistance mechanism of Alicyclobacillus acidoterrestris, label-free quantification was used to reveal some cellular changes in A. acidoterrestris during heat stress. Totally, 545 differential expression proteins were respectively identified at heat stress of 65 °C for 5 min, of which 258 proteins were up-regulated and 287 proteins were down-regulated. These significantly changed proteins were mapped to 100 pathways and some of them were mostly related to protection or repair of macromolecules such as proteins and DNA, cell wall formation, which indicated that these proteins might play crucial roles in response to heat stress. The KEGG pathway analysis combined with protein functional analysis and further validation at mRNA level suggested that A. acidoterrestris sensed the temperature rise in environment through alterations in the secondary structure of DNA and RNA molecules. The biosynthesis of antibiotics pathway and the ribosomes might be involved in signal transduction in heat stress and further trigger a large number of proteins playing a critical role in the regulation of heat stress in A. acidoterrestris. The study firstly demonstrated the global physiological response to heat stress and the results provided a better understanding of thermal adaption mechanism of A. acidoterrestris.
为了研究耐热性机制的环状芽孢杆菌，无标记定量被用来揭示一些细胞变化在耐热性芽孢杆菌在热应激。共有 545 个差异表达蛋白分别鉴定在 65°C 热应激 5 分钟，其中 258 个蛋白上调和 287 个蛋白下调。这些显著改变的蛋白被映射到 100 个途径，其中一些与蛋白质和 DNA 等大分子的保护或修复、细胞壁形成等有关，这表明这些蛋白可能在应对热应激中发挥重要作用。KEGG 途径分析结合蛋白功能分析和进一步验证在 mRNA 水平表明，耐热性芽孢杆菌通过改变 DNA 和 RNA 分子的二级结构来感知环境中的温度升高。抗生素生物合成途径和核糖体可能参与热应激中的信号转导，并进一步触发大量蛋白质在耐热性芽孢杆菌中调节热应激中发挥关键作用。本研究首次对耐热性芽孢杆菌的全局生理反应进行了研究，为耐热性芽孢杆菌的热适应机制提供了更好的理解。
