Sun Ping, Wu Jiaqi, Lin Xianrui, Wang Yi, Zhu Jianxi, Chen Chenfei, Wang Yanqiao, Jia Huijuan, Shen Jiansheng
Jinhua Academy of Agricultural Sciences, Zhejiang Institute of Agricultural Machinery, Jinhua, China.
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Front Plant Sci. 2022 Sep 15;13:967797. doi: 10.3389/fpls.2022.967797. eCollection 2022.
Phyllosphere microorganisms are closely linked to plant health. This study investigated the effect of ozonated water, mancozeb, and thiophanate-methyl on phyllosphere microorganisms in strawberry plants of the "Hongyan" variety. Sequencing analysis of the phyllosphere bacterial and fungal communities was performed using 16S rRNA gene fragment and ITS1 region high-throughput sequencing after spraying ozonated water, mancozeb, thiophanate-methyl, and clear water. Proteobacteria, Actinobacteria, and Firmicutes were the dominant bacterial phyla in strawberry. The relative abundance of Proteobacteria (82.71%) was higher in the ozonated water treatment group than in the other treatment groups, while the relative abundance of Actinobacteria (9.38%) was lower than in the other treatment groups. The strawberry phyllosphere fungal communities were mainly found in the phyla Basidiomycota and Ascomycota. The relative abundance of Basidiomycota was highest in the ozonated water treatment group (81.13%), followed by the mancozeb treatment group (76.01%), while the CK group only had an abundance of 43.38%. The relative abundance of Ascomycota was lowest in the ozonated water treatment group (17.98%), 23.12% in the mancozeb treatment group, 43.39% in the thiophanate-methyl treatment group, and 55.47% in the CK group. , , and were the dominant bacterial genera on strawberry surfaces, while , , and were the dominant fungal genera. Ozonated water was able to significantly increase the richness of bacteria and fungi and decrease fungal diversity. However, bacterial diversity was not significantly altered. Ozonated water effectively reduced the relative abundance of harmful fungi, such as , and , and enriched beneficial bacteria, such as and , more effectively than mancozeb and thiophanate-methyl. The results of the study show that ozonated water has potential as a biocide and may be able to replace traditional agents in the future to reduce environmental pollution.
叶际微生物与植物健康密切相关。本研究调查了臭氧水、代森锰锌和甲基托布津对“红颜”品种草莓植株叶际微生物的影响。在喷施臭氧水、代森锰锌、甲基托布津和清水后，利用16S rRNA基因片段和ITS1区域高通量测序对叶际细菌和真菌群落进行测序分析。变形菌门、放线菌门和厚壁菌门是草莓中的优势细菌门。臭氧水处理组中变形菌门的相对丰度（82.71%）高于其他处理组，而放线菌门的相对丰度（9.38%）低于其他处理组。草莓叶际真菌群落主要存在于担子菌门和子囊菌门。担子菌门的相对丰度在臭氧水处理组中最高（81.13%），其次是代森锰锌处理组（76.01%），而对照组仅为43.38%。子囊菌门的相对丰度在臭氧水处理组中最低（17.98%），代森锰锌处理组为23.12%，甲基托布津处理组为43.39%，对照组为55.47%。 、 和 是草莓表面的优势细菌属，而 、 和 是优势真菌属。臭氧水能够显著增加细菌和真菌的丰富度并降低真菌多样性。然而，细菌多样性没有显著改变。与代森锰锌和甲基托布津相比，臭氧水更有效地降低了有害真菌如 、 和 的相对丰度，并富集了有益细菌如 和 。研究结果表明，臭氧水具有作为杀菌剂的潜力，未来可能能够替代传统药剂以减少环境污染。
