Dehghan-Manshadi Mahsa, Azarbayjani Mohammad-Ali, Atashak Sirvan, Peeri Maghsoud, Rahmati-Ahmadabad Saleh
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Exercise Physiology, Mahabad Branch, Islamic Azad University, Mahabad, Iran.
Int J Reprod Biomed. 2022 Aug 8;20(7):549-560. doi: 10.18502/ijrm.v20i7.11557. eCollection 2022 Jul.
Hormone therapy is one of the most effective treatments for menopausal disorders, but it may increase the risk of breast cancer, coronary heart disease, and pulmonary embolism.
The present study investigated the effect of resistance training with and without vitamin D calcium(Ca ) chitosan nanoparticles on apoptosis markers in ovariectomized rats.
42 female Wistar rats were divided into 7 groups (n = 6/each). One group was assigned as the healthy controls to show the induction of menopause. The other 6 groups comprised ovariectomized (OVX) animals including: 1) vitamin D + calcium + chitosan + resistance training, 2) saline + estrogen + resistance training, 3) saline + resistance training, 4) vitamin D + calcium + chitosan, 5) saline + estrogen, and 6) OVX + control. 48 hr after the last intervention, the hippocampus tissue was extracted to measure the BCL-2-associated X (), B-cell lymphoma 2 (), and caspase-3 gene expression as well as the percentage of dead cells.
OVX rats demonstrated increased gene expression, ratio of gene expression to , caspase-3 gene expression, and percentage of dead cells of hippocampal tissue, but decreased gene expression. Resistance training and vitamin D Ca chitosan nanoparticle supplements seemed to reverse these changes.
The combination of resistance training and vitamin D Ca chitosan nanoparticle supplements may be considered a non-pharmacological treatment for OVX-induced apoptosis.
激素疗法是治疗更年期疾病最有效的方法之一，但它可能会增加患乳腺癌、冠心病和肺栓塞的风险。
本研究调查了补充和不补充维生素D钙（Ca）壳聚糖纳米颗粒的抗阻训练对去卵巢大鼠凋亡标志物的影响。
将42只雌性Wistar大鼠分为7组（每组n = 6）。一组作为健康对照组以显示更年期的诱导情况。其他6组包括去卵巢（OVX）动物，分别为：1）维生素D + 钙 + 壳聚糖 + 抗阻训练组；2）生理盐水 + 雌激素 + 抗阻训练组；3）生理盐水 + 抗阻训练组；4）维生素D + 钙 + 壳聚糖组；5）生理盐水 + 雌激素组；6）OVX + 对照组。在最后一次干预48小时后，提取海马组织以测量BCL-2相关X蛋白（）、B细胞淋巴瘤2蛋白（）和半胱天冬酶-3基因表达以及死细胞百分比。
OVX大鼠海马组织的基因表达增加、基因表达与的比值增加、半胱天冬酶-3基因表达增加以及死细胞百分比增加，但基因表达减少。抗阻训练和补充维生素D钙壳聚糖纳米颗粒似乎可以逆转这些变化。
抗阻训练与补充维生素D钙壳聚糖纳米颗粒的联合应用可被视为一种治疗OVX诱导的细胞凋亡的非药物疗法。
