Schrader W P, Pollara B
J Lab Clin Med. 1978 Oct;92(4):656-62.
A protein which specifically complexes with adenosine deaminase (complexing protein) has been localized in human kidney. Thin sections of renal tissue were treated with rabbit anti-complexing protein serum followed by fluorescein-labeled goat anti-rabbit gamma globulin serum. Complexing protein was detected in the proximal tubules of four normal kidneys. The glomeruli in sections from one of the four normal kidneys were also positive. Lung, liver, spleen, cardiac muscle, skeletal muscle, and kidney medulla were negative by this technique. The glomeruli as well as the proximal renal tubules of two patients with combined immunodeficiency disease-adenosine deaminase deficiency and three of seven patients with kidney disease contained easily detectable levels of complexing protein.
一种能与腺苷脱氨酶特异性结合的蛋白质（结合蛋白）已在人肾中定位。肾组织薄片先用兔抗结合蛋白血清处理，然后用荧光素标记的羊抗兔γ球蛋白血清处理。在四个正常肾脏的近端小管中检测到结合蛋白。四个正常肾脏之一的切片中的肾小球也呈阳性。用该技术检测，肺、肝、脾、心肌、骨骼肌和肾髓质均为阴性。两名患有联合免疫缺陷病 - 腺苷脱氨酶缺乏症的患者以及七名肾病患者中的三名患者的肾小球和近端肾小管中含有易于检测到的结合蛋白水平。
