College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China; Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, China.
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China; Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, China.
Food Res Int. 2022 Nov;161:111817. doi: 10.1016/j.foodres.2022.111817. Epub 2022 Aug 24.
Biofilms were found to promote the survival of Tetragenococcus halophilus, a functional halophilic lactic acid bacterium in the production of high-salt fermented foods under various environmental stresses including ethanol stress. Here, a comprehensive exploration of the response of T.halophilus biofilms and planktonic cells to ethanol stress was performed. Biofilms showed an ability to reduce death and damage of cell membrane and wall under 12% ethanol stress The formation of biofilm changed the characteristic of Fourier transformed infrared spectroscopy (FT-IR). RNA-seq technology and iTRAQ technology revealed the differential expression of genes and proteins in biofilm and planktonic cells with or without ethanol treatment. The differentially expressed genes and proteins played positive roles in the biosynthesis of polysaccharides, proteins, and DNA, benefitting biofilm matrix production. The shelter provided by biofilms and the differential expression of genes and proteins involved in citrate formation, malate utilization, and the biosynthesis of tryptophan, fatty acid, lipoteichoic acid, and peptidoglycan might contribute to the stress tolerance of biofilm cells together. Results presented in this study may contribute to our understanding of biofilm formation by T. halophilus and the roles of bacterial biofilm in stress tolerance.
生物膜被发现可以促进耐盐性乳酸菌四氢球菌的存活，该菌是高盐发酵食品生产中的一种功能菌，可以在包括乙醇胁迫在内的各种环境胁迫下生存。在这里，我们全面研究了 T. halophilus 生物膜和浮游细胞对乙醇胁迫的响应。生物膜显示出在 12%乙醇胁迫下降低细胞膜和细胞壁死亡和损伤的能力。生物膜的形成改变了傅里叶变换红外光谱（FT-IR）的特征。RNA-seq 技术和 iTRAQ 技术揭示了生物膜和浮游细胞在有无乙醇处理时基因和蛋白质的差异表达。差异表达的基因和蛋白质在多糖、蛋白质和 DNA 的生物合成中发挥积极作用，有利于生物膜基质的产生。生物膜提供的庇护所以及与柠檬酸形成、苹果酸利用以及色氨酸、脂肪酸、脂磷壁酸和肽聚糖生物合成相关的基因表达差异，可能共同有助于生物膜细胞的应激耐受。本研究的结果可能有助于我们理解 T. halophilus 的生物膜形成以及细菌生物膜在应激耐受中的作用。
