多个与一种新型细胞色素P-450互补DNA同源的组成型但受苯巴比妥诱导的大鼠肝细胞核RNA种类。

Multiple constitutive but phenobarbital-inducible rat hepatic nuclear RNA species homologous to a novel cytochrome P-450 cDNA.

作者信息

Foldes R L, Bresnick E

出版信息

Arch Biochem Biophys. 1987 Aug 1;256(2):534-42. doi: 10.1016/0003-9861(87)90611-4.

PMID:3619446

Abstract

A cDNA clone, pPB8, representing partial information for a phenobarbital-inducible rat hepatic cytochrome P-450, immunochemically related to cytochrome P-450b and/or P-450e, hybridized to multiple hepatic nuclear RNA species. In addition to the 3.7 +/- 0.2 kb mRNA encoding this novel cytochrome P-450 isozyme, pPB8 hybridized to nuclear RNAs of 4.9 +/- 0.3, 5.4, 5.7 +/- 0.2, and 6.3 +/- 0.1 kb. These nuclear RNAs were constitutively expressed and were inducible to various extents by phenobarbital administration. The time course of induction of these nuclear RNA components suggested product-precursor relationships. A "full-length" cDNA clone, pPB8/7, synthesized from poly(A)+ RNA homologous to pPB8, detected two mRNA species of 4.6 and 1.8 kb. The 4.6 kb nuclear RNA was inducible by 3-methylcholanthrene, Aroclor 1254, and phenobarbital, while the 1.8 kb nuclear RNA was not appreciably affected. It is suggested that pPB8 and pPB8/7 were synthesized from distinct mRNAs that share homology in their 3' regions.

摘要

一个代表苯巴比妥诱导型大鼠肝细胞色素P - 450部分信息的cDNA克隆pPB8，与细胞色素P - 450b和/或P - 450e存在免疫化学相关性，它能与多种肝细胞核RNA种类杂交。除了编码这种新型细胞色素P - 450同工酶的3.7±0.2 kb mRNA外，pPB8还能与4.9±0.3、5.4、5.7±0.2和6.3±0.1 kb的核RNA杂交。这些核RNA是组成性表达的，并且在给予苯巴比妥后会有不同程度的诱导。这些核RNA成分的诱导时间进程表明了产物 - 前体关系。一个由与pPB8同源的聚腺苷酸加尾RNA（poly(A)+ RNA）合成的“全长”cDNA克隆pPB8/7，检测到4.6和1.8 kb的两种mRNA种类。4.6 kb的核RNA可被3 - 甲基胆蒽、多氯联苯混合物Aroclor 1254和苯巴比妥诱导，而1.8 kb的核RNA则未受到明显影响。提示pPB8和pPB8/7是由在其3'区域具有同源性的不同mRNA合成的。