Bhatt B M, Bullock D W
J Reprod Fertil. 1978 Sep;54(1):177-81. doi: 10.1530/jrf.0.0540177.
Passive immunization with goat anti-rabbit uteroglobin antiserum prevents implantation in the rabbit. The dose of antiserum was too low to neutralize all of the uteroglobin present on Day 5 of pregnancy, however, and the effect could not be shown to be specific, because 'control' treatments with goat antiserum to chick avidin or normal goat serum also prevented implantation. Non-specific antisera raised in rabbits had little or no effect on implantation. Partial purification of antibodies from the non-specific goat antisera reversed their effect, while anti-uteroglobin gamma globulin still reduced implantation. Fluorescein-labelled gamma globulin fractions of anti-avidin and anti-uteroglobin both bound to blastocysts, but pure FITC-IgG showed binding only of anti-uteroglobin. Both anti-avidin and anti-uteroglobin IgG prevented implantation. It is concluded that the effect on implantation is not necessarily achieved via a specific antigen.
用山羊抗兔子宫珠蛋白抗血清进行被动免疫可阻止兔子着床。然而，抗血清的剂量过低，无法中和妊娠第5天存在的所有子宫珠蛋白，而且这种作用无法证明具有特异性，因为用山羊抗鸡抗生物素蛋白血清或正常山羊血清进行的“对照”处理也能阻止着床。在兔子体内产生的非特异性抗血清对着床几乎没有影响。从非特异性山羊抗血清中部分纯化抗体可逆转其作用，而抗子宫珠蛋白γ球蛋白仍能减少着床。抗抗生物素蛋白和抗子宫珠蛋白的荧光素标记γ球蛋白部分均能与胚泡结合，但纯异硫氰酸荧光素 - 免疫球蛋白（FITC - IgG）显示只有抗子宫珠蛋白能结合。抗抗生物素蛋白和抗子宫珠蛋白免疫球蛋白（IgG）均能阻止着床。得出的结论是，对着床的影响不一定是通过特异性抗原实现的。
