Kitajima K, Yamashita K, Fujita H
Arch Histol Jpn. 1987 Mar;50(1):113-27. doi: 10.1679/aohc.50.113.
The mechanism of follicle-like cavity formation from a monolayer of porcine thyroid cells grown in a collagen sandwich was studied. When porcine thyroid cells cultured in a monolayer on the type I collagen gel are overlaid with a second layer of collagen, changes occur in the polarity and arrangement of the cells. After 1 day of culture in the collagen sandwich, some cells in the monolayer proliferate, migrate and form two cell layers. The cell surfaces in contact with the upper or lower collagen gels always show basal characteristics. The primitive follicle lumen sealed by the zonula occludens first appears between two adjacent cells in a monolayer, or between the cells of the upper layer and those of the lower layer. Within 2 days of incubation, the cultured thyroid cells form rather flattened follicle-like cavities, even in the absence of TSH. Proliferation of the cells surrounding the lumen and luminal fusion would contribute to the development of such cavities. Bundles of numerous filaments are located along the apical and basal plasma membranes, and microtubules are markedly developed in the cytoplasm. The formation of the follicle-like cavities is inhibited by colchicine, suggesting the involvement of microtubules in the lumen-forming process. Immunohistochemistry shows that thyroid cells cultured in the collagen gel preserve the ability for thyroglobulin synthesis and basal lamina formation.
研究了在胶原三明治中生长的单层猪甲状腺细胞形成滤泡样腔的机制。当在I型胶原凝胶上单层培养的猪甲状腺细胞覆盖第二层胶原时，细胞的极性和排列会发生变化。在胶原三明治中培养1天后，单层中的一些细胞增殖、迁移并形成两层细胞。与上层或下层胶原凝胶接触的细胞表面始终呈现基底特征。由紧密连接封闭的原始滤泡腔首先出现在单层中相邻的两个细胞之间，或上层细胞与下层细胞之间。在孵育2天内，即使没有促甲状腺激素（TSH），培养的甲状腺细胞也会形成相当扁平的滤泡样腔。腔周围细胞的增殖和腔融合有助于这种腔的发育。许多细丝束沿着顶端和基底质膜定位，并且微管在细胞质中明显发育。秋水仙碱抑制滤泡样腔的形成，表明微管参与腔形成过程。免疫组织化学显示，在胶原凝胶中培养的甲状腺细胞保留了甲状腺球蛋白合成和基膜形成的能力。
