Department of Orthopedics Surgery, Public Laboratory Technology Center, Sichuan University, West China Hospital, Chengdu, China.
Eur Rev Med Pharmacol Sci. 2022 Sep;26(18):6536-6549. doi: 10.26355/eurrev_202209_29752.
The aim of our study was to determine whether abnormal hyperplasia of chondrocytes occurs in glucocorticoid-induced osteonecrosis of the femoral head (GC-ONFH) using a well-established rat model.
Rats were injected with lipopolysaccharide and methylprednisolone to induce GC-ONFH, while control animals were injected with saline (12 animals per group). Establishment of the disease model was confirmed using micro-computed tomography and hematoxylin-eosin (HE) staining of femoral head tissue sections. Chondrocyte hyperplasia was detected using HE staining and semi-quantitated using toluidine blue and saffron O staining. Expression of the autophagy marker LC3B was assessed in cartilage tissues of femoral head using immunohistochemistry.
GC-ONFH animals showed significantly greater area of abnormal chondrocyte hyperplasia in femoral head tissue sections than control animals. They also showed significantly higher expression of LC3B in articular cartilage of the femoral head.
GC-ONFH may be associated with abnormal chondrocyte hyperplasia in articular surface cartilage, which may be related to glucocorticoid-induced overactivation of autophagy.
本研究旨在使用成熟的大鼠模型来确定糖皮质激素诱导性股骨头坏死（GC-ONFH）是否存在软骨细胞异常增生。
通过向大鼠注射脂多糖和甲泼尼龙来诱导 GC-ONFH，而对照组大鼠则注射生理盐水（每组 12 只动物）。通过对股骨头组织切片进行微计算机断层扫描和苏木精-伊红（HE）染色来确认疾病模型的建立。通过 HE 染色检测软骨细胞增生，并使用甲苯胺蓝和藏红花 O 染色进行半定量分析。通过免疫组织化学评估股骨头软骨组织中自噬标志物 LC3B 的表达。
GC-ONFH 动物的股骨头组织切片中异常软骨细胞增生面积明显大于对照组动物。它们的股骨头关节软骨中 LC3B 的表达也明显升高。
GC-ONFH 可能与关节表面软骨中的异常软骨细胞增生有关，这可能与糖皮质激素诱导的自噬过度激活有关。
