Reichman M, Nen W, Hokin L E
Biochem Biophys Res Commun. 1987 Aug 14;146(3):1256-61. doi: 10.1016/0006-291x(87)90783-2.
There have been relatively few studies on the effects of neurotransmitters on the synthesis of prostaglandins in the brain. We report here that acetylcholine is very effective in stimulating prostaglandin synthesis in cerebellar cortex slices incubated in vitro, i.e., 5 microM acetylcholine increased prostaglandin levels up to 3-fold over control levels. The response was saturable and dose-dependent over the range of 0.1-5 microM acetylcholine. Atropine, at a concentration of 50 nM, abolished the response. The results indicate that high-affinity muscarinic receptors in the cerebellum are coupled to prostaglandin formation. Potassium-induced depolarization or incubation with 0.1 mM histamine also significantly increased prostaglandin formation. These findings provide support for the notion that certain neurotransmitters can modulate prostaglandin levels in the mammalian brain.
关于神经递质对大脑中前列腺素合成的影响的研究相对较少。我们在此报告，乙酰胆碱在刺激体外培养的小脑皮质切片中前列腺素合成方面非常有效，即5微摩尔乙酰胆碱可使前列腺素水平比对照水平提高多达3倍。在0.1 - 5微摩尔乙酰胆碱范围内，反应具有饱和性且呈剂量依赖性。浓度为50纳摩尔的阿托品可消除该反应。结果表明，小脑中的高亲和力毒蕈碱受体与前列腺素的形成相关联。钾诱导的去极化或与0.1毫摩尔组胺一起孵育也显著增加了前列腺素的形成。这些发现为某些神经递质可调节哺乳动物大脑中前列腺素水平这一观点提供了支持。
