Paek Seonghee, Kawai Fumihiro, Venisetty Nilamani, Kim Youngchang, Yeo Hye-Jeong
Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States.
Structural Biology Center, Argonne National Laboratory, Lemont, Illinois, United States.
Proteins. 2023 Mar;91(3):293-299. doi: 10.1002/prot.26434. Epub 2022 Oct 17.
In Gram-negative bacteria, lipopolysaccharide (LPS) is an essential component of the asymmetric outer membrane (OM). LptE is an OM lipoprotein that forms a complex with the β-barrel OM protein, LptD. Incorporation of LPS into the OM outer leaflet is essential for bacterial viability, and mediated by the LptD/E complex. The genome of Campylobacter jejuni, a major foodborne pathogen, contains over 20 putative lipoproteins including Cj1090c. Here, we report the crystal structure of Cj1090c at 2.4 Å resolution, revealing structural evidence for LptE in C. jejuni. The analysis of this crystal structure, along with the genomic context, allows us to propose the C. jejuni LPS transport system for the first time, and permits for discussion of the features of the LptD/E complex of C. jejuni.
在革兰氏阴性菌中，脂多糖（LPS）是不对称外膜（OM）的重要组成部分。LptE是一种外膜脂蛋白，它与β-桶状外膜蛋白LptD形成复合物。将LPS整合到外膜外层小叶对于细菌的生存能力至关重要，并且由LptD/E复合物介导。空肠弯曲杆菌是一种主要的食源性病原体，其基因组包含20多种假定的脂蛋白，包括Cj1090c。在这里，我们报告了Cj1090c在2.4Å分辨率下的晶体结构，揭示了空肠弯曲杆菌中LptE的结构证据。对该晶体结构的分析以及基因组背景，使我们首次提出空肠弯曲杆菌LPS转运系统，并允许讨论空肠弯曲杆菌LptD/E复合物的特征。
