Higher Institute of Biotechnology of Sidi Thabet, BVBGR-LR11ES31, University of Manouba, BiotechPôlet Sidi Thabet, Ariana, Tunisia; University of Tunis El Manar, Tunis, Tunisia.
Laboratory of Bacterial Evolution and Molecular Epidemiology, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal; Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
J Glob Antimicrob Resist. 2022 Dec;31:228-235. doi: 10.1016/j.jgar.2022.09.007. Epub 2022 Oct 3.
In this study, we aimed to assess the extent of dissemination of methicillin-resistant Mammaliicoccus sciuri in animal farms in Tunisia and evaluate the distribution of virulence and methicillin resistance genes in the M. sciuri population.
Staphylococci and mammaliicocci isolated from unhealthy animals and healthy humans from adjacent farms in Tunisia were characterized for antimicrobial susceptibility, biofilm formation, agglutination, and hemolysis abilities. Mammaliicoccus sciuri relatedness and content in antibiotic resistance and virulence genes were analyzed by whole-genome sequencing (WGS).
Mammaliicoccus sciuri was the most prevalent species (46.2%), showing the highest resistance rates to fusidic acid (94.6%), oxacillin (73%), penicillin (40.5%), clindamycin (37%), ciprofloxacin (27%), and cefoxitin (24.3%). Some isolates carried genes encoding resistance to nine different antibiotic classes. mecA was found in 35% of M. sciuri and mecC in 16.2%. All isolates carrying mecC were of S. sciuri subspecies carnaticus and carried the hybrid element SCCmec-mecC. Mammaliicoccus sciuri were able to produce strong biofilm (27%) and have clumping ability (16%). Additionally, they carried genes for capsule production (cap8, 100%), iron-regulated surface determinants (isdE, 24%; isdG, 3%), and virulence regulation (clpC and clpP, 100%). Single nucleotide polymorphisms (SNPs) analysis showed that 17 M. sciuri cross-transmission events probably occurred between different animal species and farms. Moreover, SCCmec was estimated to have been acquired five times by S. sciuri subsp. carnaticus.
Multidrug resistant and pathogenic M. sciuri were frequently disseminated between different animal species within the farm environment. mecA and mecC can be disseminated by both frequent acquisition of the SCCmec element and clonal dissemination.
本研究旨在评估耐甲氧西林 Mammaliicoccus sciuri 在突尼斯动物养殖场中的传播程度，并评估 M. sciuri 群体中毒力和耐甲氧西林基因的分布。
对来自突尼斯相邻农场的不健康动物和健康人类分离的葡萄球菌和 mammaliicocci 进行抗菌药物敏感性、生物膜形成、凝集和溶血能力的特征分析。通过全基因组测序（WGS）分析 Mammaliicoccus sciuri 相关关系及其在抗生素耐药性和毒力基因中的含量。
Mammaliicoccus sciuri 是最常见的物种（46.2%），对 fusidic acid（94.6%）、oxacillin（73%）、penicillin（40.5%）、clindamycin（37%）、ciprofloxacin（27%）和 cefoxitin（24.3%）的耐药率最高。一些分离株携带编码对 9 种不同抗生素类别的耐药性的基因。35%的 M. sciuri 携带 mecA，16.2%携带 mecC。携带 mecC 的所有分离株均为 S. sciuri 亚种 carnaticus，并携带杂交元件 SCCmec-mecC。Mammaliicoccus sciuri 能够产生强烈的生物膜（27%）和凝聚能力（16%）。此外，它们携带荚膜产生基因（cap8，100%）、铁调节表面决定因子（isdE，24%；isdG，3%）和毒力调节基因（clpC 和 clpP，100%）。单核苷酸多态性（SNP）分析显示，17 株 M. sciuri 可能在不同动物物种和农场之间发生了交叉传播事件。此外，估计 SCCmec 已经被 S. sciuri subsp. carnaticus 获得了 5 次。
多药耐药和致病性的 M. sciuri 在农场环境中经常在不同动物物种之间传播。mecA 和 mecC 可以通过频繁获得 SCCmec 元件和克隆传播进行传播。
