Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Via Amendola 122/O, 70125 Bari, Italy.
Sapienza University of Rome, Department of Chemistry, P.le Aldo Moro 5, 00185 Rome, Italy.
J Pharm Sci. 2023 Jan;112(1):243-249. doi: 10.1016/j.xphs.2022.09.029. Epub 2022 Oct 4.
We developed a simple two-dimensional/two-components theoretical model that describes the structure and functionality of a VitE-TPGS system of micelles assuming a hydrophobic inner core and an outer hydrated hydrophilic shell. We then conceptually applied the developed methodology to a simple system of VitE-TPGS micelles unloaded and loaded with an active pharmaceutical ingredient, eltrombopag, to verify if the model could reliably monitor the size change of the micelle upon loading. The fit of laboratory Small Angle X-Ray Scattering data against such model allows us to extract absolute values of the micelles size under a spherical shape hypothesis as well as the distribution within the system between components and level of hydration. The intensity scale of the SAXS experimental data needs to be normalized to a reference standard (pure water) to get absolute scattered intensities. The mathematical model which has been developed under a general hypothesis of ellipsoidal micelles, is applied to our experimental data under the simplified spherical assumption, which suitably fits our experimental data.
我们开发了一个简单的二维/两分量理论模型，用于描述含有生育酚聚乙二醇琥珀酸酯（TPGS）的胶束的结构和功能，假设胶束具有疏水性内核和外部水合亲水性外壳。然后，我们将开发的方法应用于一个简单的含有生育酚聚乙二醇琥珀酸酯（TPGS）胶束的系统，该胶束未装载和装载有活性药物成分艾曲波帕，以验证该模型是否能够可靠地监测装载时胶束的大小变化。实验室小角度 X 射线散射（SAXS）数据与该模型的拟合允许我们根据球形假设提取胶束尺寸的绝对值以及系统内组件之间的分布和水合水平。需要将 SAXS 实验数据的强度标度归一化为参考标准（纯水），以获得绝对散射强度。已经在各向同性椭球胶束的一般假设下开发的数学模型，应用于我们在简化的球形假设下的实验数据，该假设适合我们的实验数据。
