Thompson J C, Johnstone A C
Br J Exp Pathol. 1987 Jun;68(3):389-98.
Irradiated hamsters were infected with Leptospira interrogans serovar ballum in order to study the effects of impairment of the reticulo-macrophage system on the progression of the disease and the development of haemoglobinaemia. Infected and irradiated hamsters were compared with infected controls, irradiated controls and untreated controls. Red blood cells (RBCs) from untreated controls were biconcave disks while those from irradiated controls were echinocytes. Red blood cells from animals of both infected groups which were severely affected and moribund were in the form of pitted spherocytes and were associated with increased icteric indices and haemoglobinaemia. Red blood cells from less severely affected animals were echinocytic. Pitted spherocytes, however, were seen in some irradiated-infected hamsters before increased icteric indices and haemoglobinaemia were present. Both irradiated-infected and irradiated hamsters showed severe depletion of haemopoietic cells and lymphoid cells, with regeneration occurring in those animals which survived for longer. Red cell sequestration and erythrophagocytosis were present in infected-only hamsters. In irradiated-infected animals, severe anaemia was caused by both persistence of fixed macrophages, RBC sequestration and erythrophagocytosis, and haemorrhage due to vascular damage and renal papillary necrosis. A severe leucopaenia affected the irradiated hamsters' ability to suppress leptospiral multiplication resulting in the presence of larger numbers of organisms.
为了研究网状巨噬细胞系统受损对疾病进展和血红蛋白血症发展的影响，对受辐照的仓鼠感染了问号钩端螺旋体巴姆血清型。将感染且受辐照的仓鼠与感染对照、辐照对照和未处理对照进行比较。未处理对照的红细胞呈双凹圆盘状，而辐照对照的红细胞呈棘状细胞。两个感染组中受严重影响且濒死动物的红细胞呈凹陷球形，且伴有黄疸指数升高和血红蛋白血症。受影响较轻动物的红细胞呈棘状。然而，在黄疸指数和血红蛋白血症升高之前，在一些辐照感染的仓鼠中就可见到凹陷球形红细胞。辐照感染和辐照的仓鼠均出现造血细胞和淋巴细胞严重耗竭，存活时间较长的动物会发生再生。仅感染的仓鼠存在红细胞隔离和红细胞吞噬现象。在辐照感染的动物中，严重贫血是由固定巨噬细胞的持续存在、红细胞隔离和红细胞吞噬以及血管损伤和肾乳头坏死导致的出血共同引起的。严重白细胞减少影响了辐照仓鼠抑制钩端螺旋体增殖的能力，导致出现大量病原体。
