Leeksma O C, Giltay J C, Zandbergen-Spaargaren J, Modderman P W, van Mourik J A, von dem Borne A E
Br J Haematol. 1987 Jul;66(3):369-73. doi: 10.1111/j.1365-2141.1987.tb06925.x.
Recently, the synthesis by cultured human endothelial cells of a membrane protein complex immunologically related to platelet glycoprotein (GP) IIb/IIIa complex was demonstrated. Since platelet GP IIIa is known to carry the platelet alloantigen Zwa or PlA1, studies were performed to establish whether this antigen is also expressed on endothelial cells. The present report describes the results of these studies, which provide evidence for the presence of the Zwa or PlA1 antigen on the surface of cultured human endothelial cells. This evidence is based on the following observations: (1) cultured endothelial cells react with anti-Zwa (PlA1) antibodies as shown by indirect immunofluorescence; (2) two proteins are precipitated by anti-Zwa (PlA1) antibodies from lysates of 125I-labelled endothelial cells with an electrophoretic mobility corresponding with that of GP IIb and IIIa; (3) anti-Zwa (PlA1) reacts specifically, as shown by immunoblotting of sodium-dodecylsulphate polyacrylamide gels of solubilized endothelial cells, with a protein with a mobility similar to that of platelet GP IIIa.
最近，已证实培养的人内皮细胞可合成一种与血小板糖蛋白（GP）IIb/IIIa复合物具有免疫相关性的膜蛋白复合物。由于已知血小板GP IIIa携带血小板同种抗原Zwa或PlA1，因此开展了研究以确定该抗原是否也在内皮细胞上表达。本报告描述了这些研究的结果，这些结果为培养的人内皮细胞表面存在Zwa或PlA1抗原提供了证据。该证据基于以下观察结果：（1）如间接免疫荧光所示，培养的内皮细胞与抗Zwa（PlA1）抗体发生反应；（2）抗Zwa（PlA1）抗体从125I标记的内皮细胞裂解物中沉淀出两种蛋白质，其电泳迁移率与GP IIb和IIIa的电泳迁移率相对应；（3）如对溶解的内皮细胞进行十二烷基硫酸钠聚丙烯酰胺凝胶免疫印迹所示，抗Zwa（PlA1）与一种迁移率类似于血小板GP IIIa的蛋白质发生特异性反应。
