Osredkar Joško, Krivic Katja, Fabjan Teja, Kumer Kristina, Tršan Jure, Poljančič Laura, Finderle Petra, Možina Hugon
Institute of Clinical Chemistry and Biochemistry, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia.
Med Access Point Care. 2021 Oct 26;5:23992026211055095. doi: 10.1177/23992026211055095. eCollection 2021 Jan-Dec.
Although the levels of cardiac troponin I (cTnI) have proved to be a useful diagnostic biomarker of acute myocardial infarction, there are a wide variety of point-of-care (POC) analysers, which provide measurements of cTnI. The aim of this study was to compare the results obtained by the ADVIA Centaur ultra-assay cTnI assay (us-cTnI), ADVIA Centaur high-sensitive cTnI assay (hs-cTnI) and a POC high-sensitivity assay using PATHFAST. We also aimed to explore total turnaround time (TAT) for laboratory results using the POC PATHFAST analyser.
Samples from 161 patients were taken. Of these samples, 129 were tested with all three assays (us-cTnI, hs-cTnI and PATHFAST), and 32 samples were tested on PATHFAST for the comparison of whole blood, serum and plasma.
Comparison of the POC testing methods in this study demonstrated that there are strong linear relationships between all three cTnI assays (us-cTnI, hs-cTnI and POC on PATHFAST). Furthermore, we also show there are strong linear relationships between the two high-sensitive cTnI assays (hs-cTnI and PATHFAST) for blood serum samples, as determined by Passing-Bablok regression analyses. In our comparison of our new data with our older study, the TAT went down.
The timeliness of laboratory results is, in addition to accuracy and precision, one of the key indicators of laboratory performance, and at the same time has a significant impact on the course of the patient's condition. It is therefore important that the laboratory strives to meet the expectations of clinicians regarding the time from the order to the result of the analysis.
尽管心肌肌钙蛋白I（cTnI）水平已被证明是急性心肌梗死的一种有用诊断生物标志物，但有各种各样的即时检验（POC）分析仪可用于检测cTnI。本研究的目的是比较ADVIA Centaur超敏cTnI检测法（us-cTnI）、ADVIA Centaur高敏cTnI检测法（hs-cTnI）和使用PATHFAST的POC高敏检测法所获得的结果。我们还旨在探讨使用POC PATHFAST分析仪获得实验室结果的总周转时间（TAT）。
采集了161例患者的样本。其中，129份样本用所有三种检测方法（us-cTnI、hs-cTnI和PATHFAST）进行检测，32份样本用PATHFAST检测以比较全血、血清和血浆。
本研究中POC检测方法的比较表明，所有三种cTnI检测方法（us-cTnI、hs-cTnI和PATHFAST上的POC）之间存在很强的线性关系。此外，通过Passing-Bablok回归分析确定，血清样本的两种高敏cTnI检测方法（hs-cTnI和PATHFAST）之间也存在很强的线性关系。在将我们的新数据与之前的研究进行比较时，TAT缩短了。
实验室结果的及时性除了准确性和精密度外，是实验室性能的关键指标之一，同时对患者病情发展有重大影响。因此，实验室努力满足临床医生对从下达医嘱到分析结果的时间期望非常重要。
