Abe Kazuko, Kameyama Hiroki, Abe Shin-Ichi
Faculty of Health Science, Kumamoto Health Science University, Kita-ku, Kumamoto 861-5598, Japan.
Faculty of Health Science, Kumamoto Health Science University, Kita-ku, Kumamoto 861-5598, Japan,
Zoolog Sci. 2022 Oct;39(5):468-476. doi: 10.2108/zs220026.
CD34 is expressed in various cell types in various tissues/organs, and has been regarded as being expressed in progenitors in various differentiation pathways. On the other hand, morphological studies have reported the presence of a special type of interstitial cells, telocytes, which generally express CD34, and have extremely long moniliform prolongations in various tissues/organs in vertebrates. We have recently reported the successful reconstruction of testicular structures by 3-D re-aggregation culture of dissociated prepubertal mouse testicular cells, and the roles of CD34 cells in the reconstruction. However, it was unknown whether CD34 is expressed in embryonic through adult testes, and if so, in what cell type it is expressed. In order to clarify the expression of CD34 and behavior of CD34 cells during development of mouse testes, we performed immunohistochemical studies. The results show that CD34 is expressed in two cell types in testes; one is endothelial cells which co-express CD31, VE-cadherin, and integrin β1, but barely express PDGFRα and integrin α4 and α9, throughout development, while the other one is non-endothelial cells in which CD34 expression is initiated after birth, and which co-express PDGFRα and integrin α4, α9, and β1. The latter corresponds to telocytes. The present findings will lead to clarifying the roles of these two types of CD34 cells in spermatogenesis.
CD34在各种组织/器官的多种细胞类型中表达，并且被认为在各种分化途径的祖细胞中表达。另一方面，形态学研究报告称存在一种特殊类型的间质细胞，即端细胞，其通常表达CD34，并且在脊椎动物的各种组织/器官中具有极长的念珠状延长部分。我们最近报道了通过对青春期前小鼠睾丸细胞进行三维重聚集培养成功重建睾丸结构，以及CD34阳性细胞在重建中的作用。然而，尚不清楚CD34是否在胚胎期至成年期的睾丸中表达，如果表达，是在何种细胞类型中表达。为了阐明小鼠睾丸发育过程中CD34的表达及CD34阳性细胞的行为，我们进行了免疫组织化学研究。结果表明，CD34在睾丸中的两种细胞类型中表达；一种是内皮细胞，在整个发育过程中共同表达CD31、VE-钙黏蛋白和整合素β1，但几乎不表达血小板衍生生长因子受体α（PDGFRα）以及整合素α4和α9，而另一种是非内皮细胞，其CD34表达在出生后开始，并共同表达PDGFRα以及整合素α4、α9和β1。后者对应于端细胞。本研究结果将有助于阐明这两种类型的CD34阳性细胞在精子发生中的作用。
