Wang P F, Liu S H, Qian X J, Zhai X W, Wen X J, Yao M J, Zhao F M, Lu Fengmin
Department of Epidemiology and Statistics, College of Public Health Zhengzhou University, Zhengzhou 450001, China.
Fifth Medical Center of Chinese PLA General Hospital,Beijing 100039, China.
Zhonghua Gan Zang Bing Za Zhi. 2022 Aug 20;30(8):879-884. doi: 10.3760/cma.j.cn501113-20200415-00186.
To explore the diagnostic value and model of serum Golgi protein 73 (GP73) in patients with hepatitis C cirrhosis. 271 cases with chronic hepatitis C virus infection who were treated in the Fifth Medical Center of PLA General Hospital from January 2010 to December 2017 were retrospectively collected as the research objects, including 126 cases with hepatitis and 145 cases with liver cirrhosis. Serum GP73 and liver stiffness measurement (LSM) based on transient elastography test were performed in all patients. Simultaneously, blood routine, liver function, coagulation function and other related indicators were collected. GP73 diagnostic efficiency for liver cirrhosis was evaluated by receiver operating characteristic curve (ROC). GP73 diagnostic value was clarified after comparison with aspartate aminotransferase/platelet ratio index (APRI), FIB-4 index (FIB-4) and LSM. Compensated hepatitis C virus-related cirrhosis diagnostic model based on serological index was established by logistic regression analysis. The area under the receiver operating characteristic curve (AUC) of GP73, LSM, FIB-4 and APRI in the diagnosis of compensated hepatitis C virus-related cirrhosis were 0.923, 0.839, 0.836 and 0.800 respectively, and GP73 had the best diagnostic efficiency (P <0.001). LSM and GP73 combined use had improved the diagnostic sensitivity of cirrhosis to 97.24%. Multivariate logistic regression analysis revealed that GP73, age, and platelets were independent predictors of cirrhosis.Compensated hepatitis C virus-related cirrhosis diagnostic model (GAP) was established based on the result: LogitP=1/[1+exp(6.145+0.013×platelet-0.059×age-0.059×GP73)].AUC model for diagnosing compensated liver cirrhosis was 0.944, and the optimal cut-off value was 0.56, with sensitivity and specificity of 84.03% and 92.06%, respectively, and the diagnostic efficiency of this model was better than that of APRI, FIB-4, LSM and GP73 alone (<0.05). GP73 is a reliable serum biomarker for the diagnosis of compensated hepatitis C virus-related cirrhosis. The GAP diagnostic model based on GP73, platelet count, and age can further improve the diagnostic efficiency and help to diagnose patients with compensated hepatitis C virus-related cirrhosis.
探讨血清高尔基体蛋白73（GP73）在丙型肝炎肝硬化患者中的诊断价值及模型。回顾性收集2010年1月至2017年12月在中国人民解放军总医院第五医学中心接受治疗的271例慢性丙型肝炎病毒感染患者作为研究对象，其中肝炎患者126例，肝硬化患者145例。对所有患者进行血清GP73检测及基于瞬时弹性成像的肝脏硬度值（LSM）检测。同时，收集血常规、肝功能、凝血功能等相关指标。采用受试者工作特征曲线（ROC）评估GP73对肝硬化的诊断效能。将GP73与天冬氨酸氨基转移酶/血小板比值指数（APRI）、FIB-4指数（FIB-4）及LSM进行比较，明确其诊断价值。通过logistic回归分析建立基于血清学指标的代偿期丙型肝炎病毒相关性肝硬化诊断模型。GP73、LSM、FIB-4及APRI诊断代偿期丙型肝炎病毒相关性肝硬化的受试者工作特征曲线下面积（AUC）分别为0.923、0.839、0.836及0.800，GP73诊断效能最佳（P<0.001）。LSM与GP73联合使用可将肝硬化诊断敏感性提高至97.24%。多因素logistic回归分析显示，GP73、年龄及血小板是肝硬化的独立预测因素。基于上述结果建立代偿期丙型肝炎病毒相关性肝硬化诊断模型（GAP）：LogitP=1/[1+exp(6.145+0.013×血小板-0.059×年龄-0.059×GP73)]。该模型诊断代偿期肝硬化的AUC为0.944，最佳截断值为0.56，敏感性和特异性分别为84.03%和92.06%，其诊断效能优于单独的APRI、FIB-4、LSM及GP73（P<0.05）。GP73是诊断代偿期丙型肝炎病毒相关性肝硬化的可靠血清生物标志物。基于GP73、血小板计数及年龄的GAP诊断模型可进一步提高诊断效能，有助于代偿期丙型肝炎病毒相关性肝硬化患者的诊断。
