Abd-Elazeem Rana I, Abdelnabi Hend H, Hegab Doaa S, Mohamed Wesam S, Ameen Tarek E
Department of Dermatology & Venereology, Faculty of Medicine, Tanta University, Tanta, Egypt.
Department of Pediatrics, Faculty of Medicine, Tanta University, Tanta, Egypt.
Egypt J Immunol. 2022 Oct;29(4):148-155.
Interleukin-33 (IL-33) is a member of the IL-1 cytokine family and is associated with the development of different autoimmune diseases as systemic lupus erythematosus (SLE). So, the purpose of this cross-sectional study was to measure the serum IL-33 in children with SLE (c-SLE) in relation to their SLE disease activity index. This study was conducted upon 50 c-SLE patients in comparison to 50 normal matched children as a control group. Disease activity was assessed according to SLE Disease Activity Index (SLEDAI-2K). Serum IL-33 was measured by an Enzyme-linked immunosorbent assay (ELISA). Serum IL-33 was significantly higher in c-SLE patients (median: 157.47, IQR:64.49-237.57ng/l) than controls (median: 10.9, IQR: 10.04-12.51ng/L) (P= 0.001) and negatively correlated with serum C3 and C4 levels. Serum IL-33 levels were significantly higher in high disease activity status (HDAS) patients (SLEDAI-2K ≥ 10) (298.47 ± 78.84ng/l) than lupus low disease activity status (LLDAS) patients (SLEDAI-2K < 10) (112.18 ± 16.23ng/l) (P= 0.001). The receiver operating characteristic (ROC) curve analysis revealed that the best cutoff of serum IL-33 level to predict the disease activity was ≥141.3 ng/l with a sensitivity of 93%, a specificity of 90% and accuracy 97%. We concluded that serum IL-33 was higher in c-SLE patients and positively related to the disease activity index so could be used as a disease activity marker.
白细胞介素-33（IL-33）是白细胞介素-1细胞因子家族的成员，与系统性红斑狼疮（SLE）等不同自身免疫性疾病的发展有关。因此，本横断面研究的目的是测量SLE患儿（c-SLE）血清中的IL-33，并将其与SLE疾病活动指数相关联。本研究对50例c-SLE患者进行了研究，并与50例正常匹配儿童作为对照组。根据SLE疾病活动指数（SLEDAI-2K）评估疾病活动度。采用酶联免疫吸附测定（ELISA）法检测血清IL-33。c-SLE患者血清IL-33水平（中位数：157.47，四分位间距：64.49 - 237.57ng/l）显著高于对照组（中位数：10.9，四分位间距：10.04 - 12.51ng/L）（P = 0.001），且与血清C3和C4水平呈负相关。高疾病活动状态（HDAS）患者（SLEDAI-2K≥10）血清IL-33水平（298.47±78.84ng/l）显著高于狼疮低疾病活动状态（LLDAS）患者（SLEDAI-2K＜10）（112.18±16.23ng/l）（P = 0.001）。受试者工作特征（ROC）曲线分析显示，预测疾病活动的血清IL-33水平最佳截断值为≥141.3 ng/l，灵敏度为93%，特异性为90%，准确性为97%。我们得出结论，c-SLE患者血清IL-33水平较高，且与疾病活动指数呈正相关，因此可作为疾病活动标志物。
