Li Ying-Ying, Liu Hong-Chun, Zhang Qing, Feng Rui-Ting, Song Yin-Sen, Ming Liang
Translational Medicine Research Center, People's Hospital of Zhengzhou, Zhengzhou 450000, Henan Province, China.
Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, Henan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Oct;30(5):1343-1347. doi: 10.19746/j.cnki.issn.1009-2137.2022.05.007.
To investigate the effects of curcumin on the proliferation, apoptosis, and cell cycle of human acute myeloid leukemia cell line K562.
MTT method was used to detect the proliferation inhibition of logarithmic growth phase human acute myeloid leukemia K562 cells, flow cytometry was used to detect the cell cycle, Annexin V-FITC was used to detect the apoptosis rate, and real-time fluorescent quantitative PCR and Western blot were used to detect the expression of Bax, BCL-2 and caspase-3 mRNA and protein, respectively.
The inhibition rate of cell proliferation in curcumin 10, 20, and 40 μmol/L group for 24 h and 48 h were higher than that in the control group (curcumin 0 μmol/L), and the cell proliferation inhibition rate was concentration-time dependent (r=0.879, r=0.914). The proportion of G/G cells and apoptosis rate of K562 cells in the curcumin 10, 20, and 40 μmol/L group were higher than those in the control group, and showed drug concentration dependent (r=0.856, r=0.782). The expression of Bax and Caspase-3 mRNA in the curcumin 10, 20, and 40 μmol/L group was higher, while BCL-2 mRNA was lower than those in the control group, and showed drug concentration dependent (r=0.861, r=0.748, r=-0.817). The gray value of Bax protein expression in the curcumin 10, 20, and 40 μmol/L group was higher than that in the control group, while the gray value of BCL-2 and Caspase-3 protein expression was lower than that in the control group, and showed drug concentration dependent (r=0.764, r=-0.723, r=-0.831).
Curcumin can inhibit the proliferation of human acute myeloid leukemia cell line K562 cells, block the cell cycle at G/G phase, promote cell apoptosis, and induce apoptosis by regulating Bax, BCL-2, and Caspase-3.
探讨姜黄素对人急性髓系白血病细胞株K562增殖、凋亡及细胞周期的影响。
采用MTT法检测对数生长期人急性髓系白血病K562细胞的增殖抑制情况，流式细胞术检测细胞周期，Annexin V-FITC法检测凋亡率，实时荧光定量PCR和Western blot分别检测Bax、BCL-2和caspase-3 mRNA及蛋白的表达。
姜黄素10、20和40 μmol/L组作用24 h和48 h的细胞增殖抑制率均高于对照组（姜黄素0 μmol/L），细胞增殖抑制率呈浓度-时间依赖性（r=0.879，r=0.914）。姜黄素10、20和40 μmol/L组K562细胞的G/G期比例和凋亡率均高于对照组，且呈药物浓度依赖性（r=0.856，r=0.782）。姜黄素10、20和40 μmol/L组Bax和Caspase-3 mRNA表达较高，而BCL-2 mRNA低于对照组，且呈药物浓度依赖性（r=0.861，r=0.748，r=-0.817）。姜黄素10、20和40 μmol/L组Bax蛋白表达灰度值高于对照组，而BCL-2和Caspase-3蛋白表达灰度值低于对照组，且呈药物浓度依赖性（r=0.764，r=-0.723，r=-0.831）。
姜黄素可抑制人急性髓系白血病细胞株K562细胞的增殖，将细胞周期阻滞于G/G期，促进细胞凋亡，并通过调节Bax、BCL-2和Caspase-
