Gan Jun, Chen Bao-Guo, Zhang Ying, Chen Xue-Jiao
Central Laboratory, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai 317000, Zhejiang Province, China.
Central Laboratory, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai 317000, Zhejiang Province, China,E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Oct;30(5):1464-1468. doi: 10.19746/j.cnki.issn.1009-2137.2022.05.024.
To investigate the levels of cytokines in the plasma of patients with multiple myeloma(MM), and explore its clinical significance.
The levels of 6 cytokines(IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ) in the plasma of 59 newly diagnosed MM patients and 30 healthy controls were retrospectively analyzed, and the immunophenotypes were also analyzed. The plasma levels of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ were quantitatively detected by flow microsphere technology, and the differences of cytokines levels in each group were tested by Wilcoxon.
The plasma concentration of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ in MM patients were all significantly higher than those in healthy controls (P<0.05). According to the ISS staging, there were no statistically significant difference in cytokines levels of patients at each stage (P>0.05). MM patients with high CD56 expression had higher plasma levels of IL-6 than the CD56 low expression group (41.74±62.73 vs 6.31±5.60 pg/ml) (P<0.05). The plasma level of IL-6 in MM patients with high CD117 expression was higher than that in the CD56 low expression group, but there was no statistically difference (P>0.05). The plasma level of IL-6 in MM patients was significantly decreased after chemotherapy (P<0.05).
IL-6 is significantly increased in newly diagnosed MM patients, and is associated with the CD56 expression of abnormal plasma cells, which could provide important auxiliary effect on diagnosis of MM; at the same time, it is significantly decreased after chemotherapy, which may be suitable as a monitoring indicator in treatment of MM patients.
探讨多发性骨髓瘤（MM）患者血浆中细胞因子水平，并探究其临床意义。
回顾性分析59例新诊断MM患者及30例健康对照者血浆中6种细胞因子（IL-2、IL-4、IL-6、IL-10、TNF-α、IFN-γ）水平，并分析其免疫表型。采用流式微球技术定量检测IL-2、IL-4、IL-6、IL-10、TNF-α及IFN-γ血浆水平，用Wilcoxon检验比较各组细胞因子水平差异。
MM患者血浆中IL-2、IL-4、IL-6、IL-10、TNF-α及IFN-γ浓度均显著高于健康对照者（P<0.05）。根据国际分期系统（ISS）分期，各期患者细胞因子水平差异无统计学意义（P>0.05）。CD56高表达的MM患者血浆IL-6水平高于CD56低表达组（41.74±62.73 vs 6.31±5.60 pg/ml）（P<0.05）。CD117高表达的MM患者血浆IL-6水平高于CD56低表达组，但差异无统计学意义（P>0.05）。MM患者化疗后血浆IL-6水平显著降低（P<0.05）。
新诊断MM患者IL-6显著升高，且与异常浆细胞CD56表达相关，可为MM诊断提供重要辅助作用；同时化疗后显著降低，可能适合作为MM患者治疗中的监测指标。
