Division of Dentistry, Taipei Municipal Hospital, WanFang Branch, Taipei, Taiwan.
Department of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Clin Oral Investig. 2023 Mar;27(3):1207-1214. doi: 10.1007/s00784-022-04734-0. Epub 2022 Oct 8.
To explore the inflammatory and differentiation response in inflamed dental pulp cells (DPCs) induced by lipopolysaccharide (LPS) under different conditions with Biodentine and mineral trioxide aggregate (MTA) treatment.
DPCs were treated with 0.001-1 µg/mL LPS for different periods to induce inflammation. Normal and inflamed DPCs were further treated with 0.14 mg/mL Biodentine or 0.13 mg/mL MTA for different periods. mRNA expression level of IL-6, IL-8 and ALP were analysed by qPCR. DSPP protein expression was detected by western blot. The data were analysed by the Mann-Whitney test, unpaired t test or two-way ANOVA.
After treatment for different times and with different concentrations of LPS, different severity of pulp inflammation was revealed by the expressions of IL-6 and IL-8. Higher concentrations of LPS induced higher IL-6 and IL-8 expressions, and these expressions first increased and then decreased (p < 0.0001). At 96 and 192 h, Biodentine significantly suppressed IL-6 expression in both normal and inflamed DPCs (p < 0.05). At 48 and 96 h, Biodentine suppressed ALP expression in both normal and inflamed DPCs (p < 0.05). At 48 and 96 h, Biodentine induced DSPP expressions in both normal and inflamed DPCs (p < 0.05).
Biodentine enhanced more DSPP differentiation of both normal and inflamed DPCs under different treatment durations than MTA.
The prognosis of vital pulp therapy may depend on the severity of pulp inflammation which is difficult to be determined in clinical settings. Therefore, Biodentine may enhance odontogenic differentiation in different severity of pulp inflammation imply its clinical indications.
探讨不同条件下脂多糖（LPS）诱导的牙髓细胞（DPC）炎症和分化反应，以及分别用 Biodentine 和矿物三氧化物聚合体（MTA）治疗的效果。
用 0.001-1μg/mL LPS 处理 DPC 不同时间以诱导炎症，再用 0.14mg/mL Biodentine 或 0.13mg/mL MTA 处理正常和发炎的 DPC 不同时间。用 qPCR 分析 IL-6、IL-8 和 ALP 的 mRNA 表达水平，用 Western blot 检测 DSPP 蛋白表达。采用 Mann-Whitney 检验、非配对 t 检验或双因素方差分析进行数据分析。
用不同浓度 LPS 处理不同时间后，通过 IL-6 和 IL-8 的表达显示出不同严重程度的牙髓炎症。较高浓度的 LPS 诱导更高的 IL-6 和 IL-8 表达，且这些表达先增加后减少（p < 0.0001）。在 96 和 192 h，Biodentine 显著抑制正常和发炎的 DPC 中 IL-6 的表达（p < 0.05）。在 48 和 96 h，Biodentine 抑制正常和发炎的 DPC 中 ALP 的表达（p < 0.05）。在 48 和 96 h，Biodentine 诱导正常和发炎的 DPC 中 DSPP 的表达（p < 0.05）。
与 MTA 相比，Biodentine 在不同治疗时间下更能增强正常和发炎的 DPC 中更多的 DSPP 分化。
活髓治疗的预后可能取决于牙髓炎症的严重程度，而在临床环境中很难确定。因此，Biodentine 可能会增强不同牙髓炎症严重程度下的牙源性分化，暗示其临床适应证。
